短柄五加显微及薄层鉴定研究  

Microscopic and TCL identification of Acanthopanax brachypus Harms

作　　者：韦翡翡 吕蓉 何微微 晋玲 WEI Feifei;LV Rong;HE Weiwei;JIN Ling(Gansu University of Chinese Medicine College Pharmacy,Lanzhou Gansu 73OO00,China;Research Institute of Chinese (Tibetan) Medicinal Resources,Lanzhou Gansu 730000,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]中(藏)药资源研究所,甘肃兰州730000

出　　处：《中兽医医药杂志》2019年第2期43-46,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省地方药材质量标准提升研究;国家中医药管理局中药饮片标准化项目(ZYBZH-Y-GS-10);央本级重大增减支项目-名贵中药资源可持续利用能力建设(2060302)

摘　　要：目的:对短柄五加显微及薄层鉴定方法进行研究。方法:采用光学显微镜观察其组织显微特征;薄层色谱方法对其中指标成分进行鉴定。结果:短柄五加在水中反复煮,煮至沉水,软化效果较好;水粘片烤36 h时,脱片率低;染色时间为7 h染色效果较好;薄层鉴别采用硅胶GF254薄层板,以三氯甲烷-甲醇-水(10∶6∶2)10℃静置的下层溶液为展开剂展开,置紫外光灯(254 nm)下检视,有很好重现性。结论:该方法可以作为短柄五加显微及薄层鉴定方法应用于实验研究,为进一步开发利用短柄五加提供参考。objective:The microscopic methods and TLC for Acanthopanax brachypus Harms were studied. Methods:The microscopic characteristics of the tissue were observed by optical microscope,identification of indicator components by TLC.Resulst:The Acanthopanax brachypus Harms has better softening effect after repeated boiling water,when the water sticky slices are roasted 36 h,defragment rate was low,the dyeing time was better by 7 h staining. Thin layer of silica gel GF254 thin plate,the lower level solution of trichloromethane methanol water(10 ∶6 ∶2) at 10 degrees centigrade was expanded. The ultraviolet lamp(254 nm) was inspected under the UV lamp,which showed good reproducibility. Conclusion:This method could be used as a microscopic methodand TLC for Acanthopanax brachypus Harms,which provided a basis for further development and utilization of Acanthopanax brachypus Harms.

关 键 词：短柄五加 显微鉴定 薄层鉴定 实验探索 

分 类 号：S853.72[农业科学—临床兽医学]