不同年龄腰突出椎间盘组织CCNDBP1表达及意义  

EXPRESSION AND SIGNIFICANCE OF CYCLIN-D1-BINDING PROTEIN 1 IN THE INTERVERTEBRAL DISC OF PATIENTS WITH LUMBAR DISC HERNIATION AT DIFFERENT AGES

作　　者：慈延东 邱晨生 吴晓淋 方晓露 相宏飞[1] 陈伯华[1] CI Yandong;QIU Chensheng;WU Xiaolin;FANG Xiaolu;XIANG Hongfei;CHEN Bohua(Department of Spinal Surgery, The Affiliated Hospital of Qingdao University, Qingdao 266100, China)

机构地区：[1]青岛大学附属医院脊柱外科,山东青岛266100

出　　处：《青岛大学学报（医学版）》2019年第2期128-132,共5页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金项目(81802190;81772412);山东省医药卫生科技发展计划项目(2017WS033)

摘　　要：目的比较细胞周期D1型结合蛋白1(CCNDBP1)在不同年龄阶段腰椎间盘突出症病人椎间盘中的表达,探讨其与腰椎间盘退变的关系。方法收集不同年龄阶段腰椎间盘突出症病人共42例,其中30～岁、40～岁、50～岁年龄段病人各14例。取病人突出的椎间盘组织,苏木精-伊红染色观察其退变情况,采用免疫组化法、PCR法、Western印迹法从细胞、基因、蛋白水平检测CCNDBP1的表达量。结果苏木精-伊红染色结果显示,各个年龄阶段腰椎间盘突出症病人腰椎间盘组织均有不同程度退变,退变程度与年龄呈正相关。免疫组化法、PCR法和Western印迹法检测结果显示,腰椎间盘组织CCNDBP1的表达随病人年龄的增加而减少,其中30～岁年龄段病人CCNDBP1的表达显著高于40～岁年龄段病人,差异有统计学意义(F=15.42～168.30,t=3.06～6.78,P<0.05),40～岁年龄段病人CCNDBP1的表达显著高于50～岁年龄段病人,差异有统计学意义(t=2.58～9.91,P<0.05)。结论人腰椎间盘髓核组织退变过程中有CCNDBP1的参与,随着年龄的增长,CCNDBP1在退变腰椎间盘组织中的表达量逐渐减少。Objective To investigate the expression of cyclin-D1-binding protein 1 (CCNDBP1) in the intervertebral disc of patients with lumbar disc herniation at different ages and its association with lumbar disc degeneration. Methods A total of 42 patients with lumbar disc herniation at different ages were enrolled, with 14 patients each in the 30-, 40-, and 50- year groups. The prominent intervertebral disc tissue was collected, and HE staining was used to observe disc degeneration. Immunohistoche- mistry was used to measure the expression of CCNDBP1 in cells, and PCR and Western blot were used to measure the mRNA and protein expression of CCNDBP1. Results\ HE staining showed varying degrees of lumbar disc degeneration in the patients with lumbar disc herniation at different ages, and the degree of degeneration was positively correlated with age. Immunohistochemistry, PCR, and Western blot showed that the expression of CCNDBP1 in the intervertebral disc decreased with age;the 30- year group had significantly higher expression of CCNDBP1 than the 40- year group ( F =15.42-168.30, t =3.06-6.78, P <0.05), and the 40- year group had significantly higher expression of CCNDBP1 than the 50- year group ( t =2.58-9.91, P <0.05). Conclusion CCNDBP1 is involved in the degeneration of human lumbar disc nucleus pulposus, and the expression of CCNDBP1 in the degenerated intervertebral disc gradually decreases with age.

关 键 词：椎间盘移位 腰椎 细胞周期蛋白质类 年龄因素 

分 类 号：R681.53[医药卫生—骨科学]