拟南芥生态型Zu-0减少主茎分枝的遗传机理探究  

Dissection of Genetic Mechanism of Reducing Stem Branch in Zu-0 Arabidopsis Accessions

作　　者：张强[1,2] 潘芳芳[1,2] 蔡晓芸 荀道健 张宁 李鑫 黄雪清[1,2] ZHANG Qiang;PAN Fangfang;CAI Xiaoyun;XUN Daojian;ZHANG Ning;LI Xin;HUANG Xueqing(School of Life Sciences, Fudan University, Shanghai 200438, China;State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200438, China)

机构地区：[1]复旦大学生命科学学院,上海200438 [2]复旦大学遗传工程国家重点实验室,上海200438

出　　处：《复旦学报（自然科学版）》2019年第2期162-168,共7页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金(31471151)

摘　　要：植物分枝发育很大程度上决定了植物地上部的形态结构,在植物的形态建成中占有非常重要的地位.它又与作物的生物量和结实量有着密切的关系,是作物的重要农艺性状之一.以实验室常用拟南芥生态型Col-0和减少主茎分枝(RSB)的生态型Zu-0为亲本,构建了包含192个株系的重组自交系群体.利用分离群体分组分析法(BSA)检测出与RSB性状连锁的13个分子标记.最终初定位到4个减少主茎分枝的数量性状位点(QTL).qRSBZu-2和qRSBZu-3区域分别含有已知的同时控制开花时间和减少主茎分枝表型的FRI和FLC基因.qRSBZu-1和qRSBZu-4区域尽管含有已知分枝调控基因AGL6和CUC2,但AGL6和CUC2测序结果表明这2个基因在Col-0和Zu-0中没有编码氨基酸的改变,而且AGL6的表达量也没有改变,表明这2个位点还存在着其他基因参与了RSB性状的调控.构建杂合自交家系(HIF)群体,对qRSBZu-1和qRSBZu-4的真实性进行了验证.The development of shoot branching is a major determinant of plant architecture and plays a pivotal role in the morphogenesis of plant. Shoot branching is closely related to the accumulation of crop biomass and fruit yields, and is also considered as an important agronomic trait for crop. In this study, a recombinant inbred lines (RILs) population was generated from a cross between Col-0 (the commonly used Arabidopsis accessions) and Zu-0 accessions which lacks most side shoots in the axils of cauline leaves. 13 polymorphic molecular markers tightly linked to reduced stem branching phenotype (RSB) were screened out by bulked-segregant analysis (BSA). A total of 4 QTL for RSB were identified. The qRSBZu-2 and qRSBZu-3 regions respectively contain FRI and FLC genes that control flowering time and reduce the stem branch phenotype at the same time. Although qRSBZu-1 and qRSBZu-4 regions contain AGL6 and CUC2 genes that have been reported to involve the regulation of shoot branching, the sequencing results show there is no any amino acid change between Col-0 and Zu-0.In addition, there is no significant difference in the expression level of AGL6 between Col-0 and Zu-0. These results suggested there are other genes regulating the development of shoot branching in these two QTL regions. Using Heterogeneous inbred family (HIF) analysis, the qRSBZu-1 and qRSBZu-4 were validated. The results laid the foundation for further fine-mapping and map-based cloning of these two QTL.

关 键 词：拟南芥 数量性状位点 减少主茎分枝 

分 类 号：Q37[生物学—遗传学]