不同粒径色谱柱测定活血通脉片中人参皂苷类成分含量的比较  

Comparative study of ginsenosides in Huoxuetongmai Tablet by different particle size chromatographic columns

作　　者：李铮[1] 杜小伟[1] 王萌萌[1] 范妙璇[1] 于密密[1] 傅欣彤[1] 郭洪祝[1] LI Zheng;DU Xiaowei;WANG Mengmeng;FAN Miaoxuan;YU Mimi;FU Xintong;GUO Hongzhu(Beijing Institute for Drug Control, Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine, Beijing 102206, China)

机构地区：[1]北京市药品检验所中药成分分析与生物评价北京市重点实验室,北京102206

出　　处：《中国药品标准》2019年第2期149-155,共7页Drug Standards of China

基　　金：国家药品标准提高科研项目

摘　　要：目的:建立活血通脉片中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量测定方法,控制其处方中人参、三七的质量。方法:采用高效液相色谱法,使用5μm和3μm两种粒径色谱柱,以乙腈(A)-水(B)为流动相,梯度洗脱,检测波长为203 nm,柱温30℃。结果:建立的4种成分含量测定方法简便、可行;两种粒径色谱柱含量测定结果基本一致。结论:该方法准确可靠,重现性好,小粒径色谱柱方法具有经济省时的特点,可用于活血通脉片的质量控制。Objective: To establish a method for determination of four effective components of notoginsenoside R1,ginsenoside Rg1, ginsenoside Re, and ginsenoside Rb1 to control the quality of ginseng and notoginseng in Huoxuetongmai tablet. Methods: The analysis was performed in SUPELCO Discovery(R) C18(4.6 mm×250 mm, 5 μm) and SUPELCO Ascentis(R) Express C18(4.6 mm×100 mm, 2.7 μm)columns under the gradient elution of acetonitrile(A)-water(B). The detection wavelength was set at 203 nm, and the column temperature was 30 ℃. Results:The established method for determination of four components was easy and feasible. The test results obtained with different particle size columns were similar. Conclusion:This accurate, reliable and reproducible method can be used to control the quality of ginseng and notoginseng in Huoxuetongmai Tablet.

关 键 词：活血通脉片 粒径 含量测定 三七皂苷R1 人参皂苷Rg1 人参皂苷RE 人参皂苷RB1 

分 类 号：R921.2[医药卫生—药学]