红树莓根茎水煎剂对S180荷瘤小鼠肿瘤组织中C-myc、Cath-D表达的影响  

Effects of raspberry rhizome decoction on expression of C-myc and Cath-D in tumor tissues of S180 tumor-bearing mice

作　　者：孙玉珍[1] 方毅[2] 于晓红[1] 颜培宇[1] SUN Yu-zhen;FANG Yi;YU Xiao-hong;YAN Pei-yu(Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang Province,China;Suihua Aier Ophthalmological Hospital,Suihua 152000,Heilongjiang Province,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]绥化爱尔眼科医院,黑龙江绥化152000

出　　处：《海南医学院学报》2019年第9期641-644,649,共5页Journal of Hainan Medical University

基　　金：黑龙江省教育厅经费资助项目;黑龙江中医药大学科研基金资助项目(201734)~~

摘　　要：目的:探讨红树莓根茎水煎剂(RRD)对S180荷瘤小鼠核蛋白类调控基因(C-myc)、组织蛋白酶D(Cath-D)在肿瘤组织中表达的影响,分析RRD的抗肿瘤作用。方法:取40只清洁级ICR健康昆明(KM)种小鼠,雌性和雄性各占一半,随机分为4组,模型组、中药组(RRD)、环磷酰胺组(CTX)及联合用药组(RRD+CTX),每组10只,在腋下位置皮下接种S180瘤株细胞悬液0.2 mL造模。24 h后开始给药,模型组:生理盐水灌胃每天0.02 mL/g;RRD组:RRD灌胃每天0.4 mL/20 g;CTX组:生理盐水灌胃每天0.4 mL/20 g,腹腔注射CTX每天20 mg/kg;RRD+CTX组:RRD灌胃每天0.4 mL/20 g,腹腔注射每天CTX 20 mg/kg,1次/d,连续10 d。用免疫组化法检测肿瘤组织C-myc、Cath-D表达含量。结果:RRD能够使S180荷瘤小鼠肿瘤组织C-myc、Cath-D表达明显下降,且与模型组比较,差异有统计学意义(P<0.05)。结论:RRD对S180荷瘤小鼠的肿瘤抑制作用可能与抑制肿瘤增殖、侵袭和转移相关蛋白C-myc、Cath-D的表达有关。Objective: To investigate the effects of RRD on the expression of nuclear protein regulatory genes(C-myc) and cathepsin D(Cath-D) in tumor tissues of S180 tumor-bearing mice, and to analyze the anti-tumor effect of RRD. Methods: Forty clean ICR healthy Kunming(KM) mice were randomly divided into four groups: model group, traditional Chinese medicine group(RRD), cyclophosphamide group(CTX) and combined drug group(RRD + CTX). Ten mice in each group were subcutaneously inoculated with S180 cell suspension at the axillary position to establish the model. After 24 h, the model group was given orally 0.02 mL/g/d with normal saline;RRD group was given orally 0.4 mL/20 g/d with RRD;CTX group was given orally 0.4 mL/20 g/d with normal saline and intraperitoneally 20 mg/kg/d with CTX;RRD+CTX group was given orally 0.4 mL/20 g/d with RRD and intraperitoneally 20 mg/kg/d with CTX once a day for 10 d. The expression of C-myc and Cath-D in tumor tissues was detected by immunohistochemistry. Results: RRD could significantly decrease the expression of C-myc and Cath-D in the tumor tissues of S180 tumor-bearing mice, and there was a significant difference between RRD and model group(P<0.05). Conclusion: The inhibitory effect of RRD on S180 tumor-bearing mice may be related to the inhibition of the expression of C-myc and Cath-D related proteins in tumor proliferation, invasion and metastasis.

关 键 词：红树莓根茎水煎剂 S180瘤株 C-MYC CATH-D 

分 类 号：R285.5[医药卫生—中药学]