不同产地知母药材的含量测定及指纹图谱研究  

Determination and fingerprint of Rhizoma Anemarrhena from different habitats

作　　者：陈素娟 张艳秋 聂静 杨芊芊 高歌 张旗 韩晶晶 蔡程科[1] 李强[1] CHEN Sujuan;ZHANG Yanqiu;NIE Jing;YANG Qianqian;GAO Ge;ZHANG Qi;HAN Jingjing;CAI Chengke;LI Qiang(School of Traditional Chinese Medicine,Beijing University of Traditional Chinese Medicine,Beijing 102488,China;Natural Pharmaceutical Chemistry Department,School of Pharmacy,Fujian Medical University,Fuzhou 350122,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]福建医科大学药学院天然药物学系,福州350122

出　　处：《西北药学杂志》2019年第3期285-288,共4页Northwest Pharmaceutical Journal

基　　金："重大新药创制"科技重大专项(编号:2011ZX09201-201-15)

摘　　要：目的收集不同产地的知母药材,测定其芒果苷和知母皂苷BⅡ的含量,建立知母药材的HPLC-UV-ELSD指纹图谱测定方法。方法采用HPLC-UV-ELSD法测定3个不同产地的15批知母药材。色谱条件:Agilent ZORBAX SB-C_(18)色谱柱(250 mm×4.6 mm,5μm)。乙腈(A)-1 mL·L^(-1)乙酸(B)梯度洗脱,梯度为:0～5 min,2%A→5%A;5～12 min,5%A→20%A;12～18 min,20%A→25%A;18～23 min,25%A;23～40 min,25%A→100%A;40～45 min,100%A。柱温:30℃;流速:1.0 mL·min^(-1);波长:258 nm。ELSD参数:漂移管温度:105℃;氮气流速:2.6 L·min^(-1)。结果 15批知母药材中芒果苷的含量为0.803%～1.687%,知母皂苷BⅡ的含量为3.253%～7.332%。建立了知母药材的指纹图谱,包含11个共有峰,通过对照品指认了芒果苷和知母皂苷BⅡ2个峰,对15批知母药材指纹图谱的相似度进行评价,其相似度均大于0.9。结论该方法操作简便、快捷,可用于知母药材的质量研究。Objective To determine the contents of mangiferin and saponins BⅡ of Rhizoma Anemarrhena from different origins,and to establish a method for the determination of HPLC-UV-ELSD fingerprint of Rhizoma Anemarrhena . Methods15 batches of Rhizoma Anemarrhena from 3 different producing areas were determined by HPLC-UV-ELSD.The chromatographic conditions were as follows:Agilent ZORBAX SB-C18 chromatographic column (250 mm×4.6 mm,5 μm).Acetonitrile (A)-0.1 mL ·L^-1 acetic acid (B) gradient elution.The gradient was 0-5 min,2%A →5%A;5-12 min,5%A→20%A;12-18 min,20%A→25%A;18-23 min,25%A;23-40 min,25%A→100%A;40-45 min,100%A.The column temperature was 30 ℃, the flow rate was1.0 mL ·min^-1 , and the wavelength was 258 nm.ELSD parameters were as follows:drift tube temperature105 ℃,nitrogen flow rate 2.6 L·min^-1 . Results The content of mangiferin in15 batches of Rhizoma Anemarrhena was 0.803%-1.687%,and the content of saponins BⅡ was 3.253%-7.332%.The fingerprint of the medicinal herb of Rhizoma Anemarrhena was established,including11 common peaks,and 2 were identified by the control products,namely,mangiferin and saponins BⅡ respectively.The similarity of the fingerprints of the15 batches of Rhizoma Anemarrhena was evaluated,the similarity of which was more than 0.9. Conclusion The method is simple and quick,and can be used for the quality research of Rhizoma Anemarrhena .

关 键 词：知母 芒果苷 知母皂苷BⅡ 含量测定 指纹图谱 

分 类 号：R284[医药卫生—中药学]