比卡鲁胺联合塞来昔布对前列腺癌细胞LNCaP增殖的影响  

Effects of bicalutamide combined with celecoxib on the proliferation of prostate cancer cells LNCaP

作　　者：白晓黎[1] 王丽[1] 谢秀娟[1] 陈宏伟 BAI Xiaoli;WANG Li;XIE Xiujuan;CHEN Hongwei(Department of Drug Dispensing,the First Affiliated Hospital of He′nan University of Science and Technology,Luoyang471003,China;Luoyang Central Hospital Affiliated to Zhengzhou University,Luoyang 471000,China)

机构地区：[1]河南科技大学第一附属医院药品调剂科,洛阳471003 [2]郑州大学附属洛阳中心医院,洛阳471000

出　　处：《西北药学杂志》2019年第3期368-371,共4页Northwest Pharmaceutical Journal

摘　　要：目的观察比卡鲁胺联合塞来昔布对前列腺癌细胞LNCaP增殖的影响并探讨其可能的作用机制。方法培养前列腺癌LNCaP细胞,采用MTT法测定细胞生长的抑制情况,化学发光法检测细胞培养液中总前列腺特异抗原(PSA)的浓度,流式细胞仪检测各组细胞的凋亡率,Western Blot检测各组细胞Caspase 3和Caspase 8蛋白的表达情况。结果 MTT检测结果显示,对照组在24,48和72 h的细胞生长抑制率均为0。比卡鲁胺组、塞来昔布组和联合组在24,48和72 h的生长抑制率均明显较高(P<0.05)。对组间细胞的生长抑制率联合组显著优于单一组(P<0.05)。化学发光法检测结果显示,与对照组相比,比卡鲁胺组、塞来昔布组以及联合组PSA浓度明显降低,其中联合组对PSA的抑制程度明显优于比卡鲁胺组和塞来昔布组(P<0.05)。流式细胞仪检测结果显示,对照组在24,48和72 h的细胞凋亡率均低于比卡鲁胺组、塞来昔布组和联合组在24,48和72 h的生长抑制率。组间细胞的生长抑制率联合组显著优于比卡鲁胺组和塞来昔布组(P<0.05)。Western Blot结果提示,与对照组相比,给药组Caspase 3和Caspase 8蛋白的表达水平均显著升高,且联合组明显高于比卡鲁胺组和塞来昔布组(P<0.05)。结论比卡鲁胺联合塞来昔布能有效抑制前列腺癌细胞LNCaP的增殖,其机制可能与促进相关蛋白Caspase 3和Caspase 8凋亡进而促进肿瘤细胞的凋亡有关。Objective To observe the effects of bicalutamide combined with celecoxib on the proliferation of prostate cancer cells LNCaP and explore its possible mechanism. Methods Prostate cancer LNCaP cells were cultured,and MTT assay was used to determine the inhibition of cell growth.The concentration of total prostate specific antigen (PSA) in cell culture medium was measured by chemiluminescence,and the apoptosis rate of each group of cells was detected by flow cytometry.The expression of Caspase 3 and Caspase 8 proteins in each group of cells was detected by Western Blot. Results The results of MTT assay showed that the growth inhibition rate of the control group was 0 at 24,48 and 72 h.Compared with the control group,bicalutamide group, celecoxib group and the combination group had significantly higher growth inhibition rates at 24,48 and 72 h ( P <0.05).The growth inhibitory rate of the cells was further compared among the groups.The combined group was significantly better than the single group ( P <0.05).The results of chemiluminescence assay showed that the concentration of PSA in bicalutamide,celecoxib,and combination groups was significantly reduced compared with the control group,and the combined group had significantly better inhibition of PSA than bicalutamide or celecoxib group ( P <0.05).The flow cytometry results also showed that the apoptosis rate of the control group at 24,48 and 72 h was lower than that of the bicalutamide group.The growth inhibitory rate of the cells was further compared among the groups.The combined group was significantly better than the bicalutamide group or celecoxib group ( P <0.05).Western Blot results showed that compared with the control group,the expression levels of Caspase 3 and Caspase 8 protein in the drug administration group were significantly increased,and the expression levels of Caspase 3 and Caspase 8 protein in the combination group were significantly higher than those in the bicalutamide or celecoxib groups ( P <0.05). Conclusion Bicalutamide combined with celecoxi

关 键 词：比卡鲁胺 塞来昔布 前列腺癌 增殖 

分 类 号：R965[医药卫生—药理学]