金樱子总黄酮的不同萃取组分高效液相色谱法分析  

High performance liquid dromatography analysis of different extraction components of flavonoids from Lavigate Rosae Fructus

作　　者：吴剑峰 李峰[2] 陈传平[2] 翟金霞 WU Jian-feng;LI Feng;CHEN Chuan-ping;ZHAI Jin-xia(Basic Medical School of Anhui Medical University,Hefei Anhui 230000;Department of Clinical Medicine of West Anhui Healthy Vocational College,Lu'an Anhui 237005,China)

机构地区：[1]安徽医科大学基础医学院,安徽合肥233000 [2]皖西卫生职业学院临床医学系,安徽六安237005

出　　处：《蚌埠医学院学报》2019年第4期495-499,共5页Journal of Bengbu Medical College

摘　　要：目的:探讨金樱子总黄酮的不同萃取组分的黄酮含量。方法:以金樱子为原料,用60%乙醇取金樱子总黄酮,分别用石油醚、三氯甲烷、乙酸乙酯、正丁醇萃取,回收有机溶剂获石油醚、三氯甲烷、乙酸乙酯、正丁醇及水等5个萃取相。采用高效液相色谱-紫外检测法测定各相在不同波长下的峰值,并采用高效液相色谱-二极管阵列检测法分析各相中峰是否为黄酮。结果:结果显示小极性黄酮主要分布在三氯甲烷相中,占总黄酮的47.72%;大极性黄酮主要分布在正丁醇相中,占总黄酮的44.07%;而水相中有少量黄酮,占总黄酮的9.17%;石油醚相和乙酸乙酯相黄酮含量极少。结论:金樱子黄酮类成分主要富集于三氯甲烷相和正丁醇相。Objective:To analyze the flavonoids content in different extraction components from Laevigatae Rosae Fructus. Methods: The crude flavonoid was extracted from Rosae Laevigatae Fructus using 60% ethanol(v:v),and extracted using petroleum ether, chloroform,ethyl acetate and n-butanol. The five phases of petroleum ether, chloroform, ethyl acetate, n-butanol and water were obtained,the peak value of each phase was measured using HPLC-UV,and the peak of each phase was analyzed by HPLC-DAD. Results:The low polar flavonoids mainly distributed in the chloroform phase,which accounted for 47. 72% of the total flavonoids. The high polar flavonoids mainly distributed in the n-butanol phase,which accounted for 44. 07% of the total flavonoids. The small amount of flavonoids in water phase was identified,which accounted for 9. 17% of the total flavonoids. The very small amount of flavonoids in petroleum ether and ethyl acetate phases was found. Conclusions:The flavonoids of Laevigate Rosae Fructus mainly distributes in the phase of trichloromethane and n-butanol.

关 键 词：金樱子 总黄酮 萃取 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]