盐碱胁迫下一株促进苜蓿生长的细菌筛选与鉴定  

Screening and identification of Medicago Sativa Linn growthpromoting rhizobacteria under saline-alkali stress

作　　者：向君亮 唐呈瑞 王佳琦 刘权[1] 张兴梅[3] 殷奎德[1] XIANG Jun-liang;TANG Cheng-rui;WANG Jia-qi;LIU Quan;ZHANG Xing-mei;YIN Kui-de(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China;Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang,Liaoning 110016,China;College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [3]黑龙江八一农垦大学农学院,黑龙江大庆163319

出　　处：《干旱地区农业研究》2019年第2期266-272,共7页Agricultural Research in the Arid Areas

基　　金：大庆市科技局资助项目(Zd-2017-60)

摘　　要：为改良和利用盐碱地,本研究以大庆草原盐碱土为材料,在Na_2CO_3和NaHCO_3终浓度分别为50、100 mmol·L^(-1),pH 8.5的条件下对其中耐盐碱细菌进行分离,并在CAS铁载体检测培养基、ADF培养基、PKO无机磷培养基等功能性培养基上对菌株的促生功能进行检测;利用16S rDNA序列分析法进行菌株的菌种鉴定,在pH 8.5,25 mmol·L^(-1) Na_2CO_3胁迫下测试菌株对苜蓿种子发芽率、发芽势及幼苗生长的影响。结果表明:筛选得到的菌株S11具有产植物激素IAA和产ACC脱氨酶功能;通过对菌株16S rDNA序列进行分析,初步鉴定菌株S11为堀越氏芽孢杆菌(Bacillus horikoshii)。盐碱胁迫条件下,浸种接菌处理苜蓿种子后,种子发芽率和发芽势分别提高了15%和10%,幼苗根长、株高、鲜重以及叶绿素含量分别增加了34.78%、12.5%、27.3%和43.30%,这证明菌株S11具有缓解盐碱胁迫促进苜蓿生长的功能。In order to ameliorate and utilize saline-alkali soil,the saline-alkali soil was used to separate the saline-alkali-tolerant bacteria under pH 8.5,50 mmol·L^-1 Na2CO3 and 100 mmol·L^-1 NaHCO3.The growth-promoting function of the strain was tested by CAS,ADF,PKO and other functional medium.The 16S rDNA sequence analysis was used to identify the strains.The effect of the bacteria on the alfalfa seed germination rate,germination potential and seedling growth were investigated under pH 8.5 and 25 mmol·L^-1 Na2CO3.The results showed that strain S11 could produce IAA and ACC deaminase,and it was identified as Bacillus horikoshii.Under the saline-alkali condition and after inoculation of strain S11,alfalfa seed germination rate and germination potential were increased by 15% and 10% ,respectively.The root length,plant height,fresh biomass,and chlorophyll content were increased by 34.78% 、12.5% 、27.3% and 43.30% ,respectively.This proved that strain S11 could relieve saline-alkali stress and promote the growth of alfalfa.

关 键 词：耐盐碱促生菌 菌株筛选鉴定 盐碱土 苜蓿 

分 类 号：S182[农业科学—农业基础科学] S432.4