HIF-1α蛋白在烧伤后增生性瘢痕组织中的表达及临床意义  

Expression and Clinical Significance of HIF-1α Protein in the Tissues with Hypertrophic Scar after Burn

作　　者：周荣华 薛强东 吴丽芳 王启操 张雅洁 李青 ZHOU Rong-hua;XUE Qiang-dong;WU Li-fang;WANG Qi-cao;ZHANG Ya-jie;LI Qing(Department of Dermatology,Longhua Branch of Shenzhen People's Hospital,Shenzhen 518109,Guangdong,China)

机构地区：[1]深圳市人民医院龙华分院皮肤科,广东深圳518109

出　　处：《中国美容医学》2019年第5期73-76,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨烧伤后瘢痕增生患者组织中HIF-1α蛋白的表达水平及其临床意义。方法:选取82例烧伤后瘢痕增生患者的增生性瘢痕组织作为实验组,同时取其自身正常皮肤组织为对照组。采用荧光定量PCR检测HIF-1α的mRNA表达水平;采用免疫组化检测HIF-1α的蛋白表达水平;采用Westernblot检测HIF-1α相关细胞因子,转化生长因子β(TGF-β)、E-钙黏蛋白(E-cadherin)及血管内皮生长因子(VEGF)的蛋白表达水平。结果:与正常组织(0.29±0.03)相比,增生性瘢痕组织中HIF-1α的mRNA相对表达水平(1.45±0.16)显著增高,差异有统计学意义(P<0.05);免疫组化显示,与正常组织中HIF-1α的阳性表达率(8.54%)相比,增生性瘢痕组织中HIF-1α的阳性表达率(70.7%)显著升高(P<0.05);Western blot显示,与正常组织相比,增生性瘢痕组织中HIF-1α、TGF-β及VEGF蛋白表达水平显著增加,E-cadherin蛋白表达水平显著下降,差异有统计学意义(P<0.05)。结论:HIF-1α在增生性瘢痕组织中高表达,并通过调控下游细胞因子TGF-β、VEGF及E-cadherin的表达参与增生性瘢痕的发生、发展。Objective To investigate the expression and clinical significance of HIF-1 α protein in burn scar hyperplasia. Methods The hyperplastic scar tissue of 82 patients with scar hyperplasia after burn was selected as experimental group, and its normal skin tissue was taken as control group. The HIF-1α mRNA expression level was detection by real-time PCR. The protein expression of HIF-1α was analyzed by immunohistochemistry. The expression of HIF-1α-related cytokines TGF-β, E-cadherin and VEGF were detected by Western blot. Results Compared with normal tissues(0.29±0.03), the mRNA relative expression of HIF-1α in hypertrophic scar tissue(1.45±0.16)was significantly increased (P <0.05). Immunohistochemistry showed that the positive expression of HIF-1α in hypertrophic scar tissue (70.7%) was significantly higher than that in normal tissues (8.54%,P <0.05). Western blot showed that compared with normal tissues, the expression of HIF-1α, TGF-β and VEGF in hypertrophic scar tissue were significantly increased, whereas the expression level of E-cadherin was significantly decreased (P <0.05). Conclusion HIF-1α was highly expressed in hypertrophic scar tissue and participates in the development of hypertrophic scar by regulating the expression of downstream cytokines TGF-β, VEGF and E-cadherin.

关 键 词：增生性瘢痕 HIF-1Α TGF-β VEGF E-CADHERIN 蛋白表达 

分 类 号：R619.6[医药卫生—外科学]