封丘金银花炭疽病病原的分离和鉴定  

Isolation and identification of honeysuckle anthracnose pathogen in Fengqiu

作　　者：李志红 翟凤艳[1] 张永霞 王迪 邢登可 鲁海菊[2] 刘英杰[1] LI Zhihong;ZHAI Fengyan;ZHANG Yongxia;WANG Di;XING Dengke;LU Haiju;LIU Yingjie(School of Resource and Environmental Sciences,Henan Institute of Science and Technology,Xinxiang 453003,China;Department of Life Science and Technology,Honghe College,Mengzi 661199,China)

机构地区：[1]河南科技学院资源与环境学院,河南新乡453003 [2]红河学院生命科学与技术学院,云南蒙自661199

出　　处：《河南科技学院学报（自然科学版）》2019年第2期20-23,共4页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金：国家大学生创新训练项目(2018CX29);河南省高等学校青年骨干教师培养计划项目(2016GGJS109)

摘　　要：为明确河南封丘金银花炭疽病病原种类,对采自封丘的金银花炭疽病病叶,依据柯赫氏法则进行分离、纯化、回接和再分离,证明获得的分离菌株是金银花炭疽病的致病菌.对其培养性状、在寄主上的形态特征及萌发方式进行观察,并对其r DNA-ITS序列进行分析,结果表明:致病菌株在PDA培养基上菌落呈白色,后期菌落中央颜色变暗,生分生孢子盘;分生孢子盘具黑色刚毛,分生孢子长圆柱形,两端钝圆,单细胞.核糖体DNA-ITS序列与GenBank数据库胶孢炭疽菌同源性达99%.将封丘金银花炭疽病病原菌鉴定为胶孢炭疽菌Colletotrichum gloeosporioides.In order to determine the pathogen of Lonicera japonica anthracnose in Henan Province,infected leaves were collected from Fengqiu,Henan Province.The pathogen was isolated,back-inoculated,re-isolated by Koch’s postulates and was identified according to morphological and culture characteristics and the sequence of ribosomal DNA-ITS.The results showed that the fungus colonies on PDA were white,later pale yellow.Acervuli with setae formed in the center of the colonies.Conidia long cylindrical,obtuse at both ends,single-celled.rDNA-ITS sequence analysis indicated that the pathogen and Colletotrichum gloeosporioidesisolate COL4 were 99%in homology.he pathogen was identified as Colletotrichum gloeosporioides.

关 键 词：金银花 炭疽病 胶孢炭疽菌 rDNA-ITS序列 鉴定 

分 类 号：S567.79[农业科学—中草药栽培]