电感耦合等离子体质谱仪在血流感染早期诊断中的初步应用  

Preliminary application of inductively coupled plasma mass spectrometer in early diagnosis of bloodstream infection

作　　者：赵苏瑛[1] 蒋维[1] 吴倩[2] ZHAO Suying;JIANG Wei;WU Qian(the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing,Jiangsu 210029,China;School of Public Health,Nanjing Medical University,Nanjing,Jiangsu 210029,China)

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]南京医科大学公共卫生学院,江苏南京210029

出　　处：《国际检验医学杂志》2019年第9期1081-1085,1089,共6页International Journal of Laboratory Medicine

摘　　要：目的使用电感耦合等离子体质谱仪(ICP-MS)研究血流感染患者血清中的金属元素水平,探讨金属元素在血流感染患者血清中的浓度变化以及和感染病原菌之间的相关性,获得差异表达的金属元素图谱,评价将此类质谱仪应用于血流感染早期诊断的可行性。方法收集血流感染患者的血清作为血流感染组,同时分离出血液中的病原菌,按病原菌种类将标本分类,选用健康者血清作为阴性对照组。运用ICP-MS测定患者血清中各类金属元素含量的变化,使用主成分分析(PCA)和偏最小二乘法判别分析(PLS-DA)绘制金属元素图谱,寻找出浓度明显改变的金属元素,组间各金属元素的相关性研究使用Pearson相关性分析。结果一共建立了4个病原菌血流感染的金属元素图谱(包含金黄色葡萄球菌、大肠埃希菌、粪肠球菌和肺炎克雷伯菌),血流感染组与对照组间有浓度明显改变的金属元素,包括钯、金、钍、锡等;Pearson相关性分析显示这些金属元素与血流感染和感染菌种类之间均有相关性,如钙与金黄色葡萄球菌血流感染呈负相关性(r=-0.788),钯和金均与大肠埃希菌的血流感染呈正相关性(r=0.906、0.748)。结论从上述研究结果看,具有差异性的金属元素图谱可以作为血流感染早期诊断、预防和预后的潜在标志物,其应用性值得进一步的深入研究。Objective To study the levels of metal elements in blood stream infection patients by Inductively coupled plasma mass spectrometer (ICP-MS),to explore the changes of concentration of metal elements in blood stream infection patients′serum and the correlation between metal elements and infectious pathogens,to obtain differential expression of metal elements maps,and to evaluate the feasibility of applying this kind of mass spectrometer to early diagnosis of blood stream infection. Methods Serum samples from patients with bloodstream infection were collected as bloodstream infection group,and pathogens in blood stream were isolated.Samples were classified according to the types of pathogens.Serum from healthy persons was selected as negative control.ICP-MS was used to determine the changes of concentration of various metal elements in serum of patients.principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to draw the metal element atlas,and the metal elements with obvious changes in concentration were found.Pearson correlation analysis was used to study the correlation of various metal elements between groups. Results The metal element profiles of four pathogenic bacteria in bloodstream infection (Staphylococcus aureus,Escherichia coli,Enterococcus faecium,and Klebsiella pneumonia) were established.Metal elements,including palladium,gold,thorium and tin,changed significantly between the bloodstream infection group and the control group.Pearson correlation analysis showed that these metal elements were correlated with bloodstream infections and types of infectious bacteria.For instance,calcium was negatively correlated with blood stream infection of Staphylococcus aureus ( r =-0.788),and palladium and gold were positively correlated with blood stream infection of Escherichia coli ( r =0.906,0.748). Conclusion The research results showed that differential maps of metal elements can be used as potential markers for early diagnosis,prevention and prognosis of bloodstrea

关 键 词：金属组学 金属元素 血流感染 电感耦合等离子体质谱仪 

分 类 号：R446.1[医药卫生—诊断学]