具有抗凝血活性的聚氨基酸的合成及性能研究  

Synthesis and properties of polyamino acids with anticoagulant activity

作　　者：路德待[1] 王相雅 金媛湲 LU De-dai;WANG Xiang-ya;JIN Yuan-yuan(Key Laboratory of Eco-Environment-Related Polymer Materials of the Ministry of Education, College of Chemistry and Chemical Engineering,Northwest Normal University,Lanzhou 730070,Gansu,China)

机构地区：[1]西北师范大学化学化工学院生态环境相关高分子材料教育部重点实验室,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2019年第3期79-85,共7页Journal of Northwest Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(21865029);中国科学院2018年度"西部青年学者"项目(人字[2018]99号)

摘　　要：通过亲核试剂三乙烯四胺(TETA)引发L-酪氨酸和L-谷氨酸的α-氨基酸N-环内酸酐(NCAs)的活性可控开环聚合(ROP),并进行氯磺酸酯化修饰,制备了一种具有抗凝血活性的磺酸酯化聚(L-酪氨酸-co-L-谷氨酸)(PTG-SO_3),并对PTG-SO_3的结构、酶促降解性能、细胞毒性以及血液相容性等进行了测试.结果表明,合成产物为PTG-SO_3,具有良好的生物降解性(16 d后可降解约65%)、低细胞毒性(培育72 h后,细胞活性约为70%)、良好的抗凝血性能和血液相容性(活化的部分凝血活酶时间延长约37 s).该聚合物有望应用于生物医学领域.Using the nucleophilic reagent triethylenetetramine (TETA) as initiator, L-tyrosine and L-glutamic acid alpha amino acid N-carboxylic acid anhydride (NCAs) were actively controlled to occur the ring opening polymerization(ROP).Then,esterification modification of chlorosulfonic acid was prepared,a sulfonic acid esterification poly(L-tyrosine-co-L-glutamic acid)(PTG-SO 3) with anticoagulant activity had been prepared.The structure of PTG-SO 3,enzymatic degradation performance,cytotoxicity and blood compatibility were tested.The results showed that the product is PTG-SO 3 which has good biodegradability (about 65% biodegradable 16 d later),low cytotoxicity ( after 72 h of culture,the cell activity is about 70%),favourable anticoagulant energy and blood compatibility( activated partial thrombin time can be extended by about 37 s).The polymer is expected to be used in biomedical applications.

关 键 词：聚氨基酸 肝素模拟物 抗凝血高分子 

分 类 号：O631[理学—高分子化学] TQ460.1[理学—化学]