蛛网萼未成熟种子培养与离体繁殖  

Immature seed culture and in vitro propagation of Platycrater arguta

作　　者：曹丽雯 支肖 戴嘉禾 于宁宁 陈怡倩 叶立新 陈利萍[1] 徐礼根 CAO Liwen;ZHI Xiao;DAI Jiahe;YU Ningning;CHEN Yiqian;YE Lixin;CHEN Liping;XU Ligen(Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;Fengyangshan Administration Office, Fengyangshan- Baishanzu National Nature Reserve, Lishui 323700, Zhejiang, China)

机构地区：[1]浙江大学农业与生物技术学院园艺系,杭州310058 [2]浙江凤阳山-百山祖国家级自然保护区凤阳山管理处,浙江丽水323700

出　　处：《浙江大学学报（农业与生命科学版）》2019年第2期157-163,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：农业农村部"引进国际先进农业技术"项目(2011-G31);浙江省科技计划项目(2019C02026);浙江省重大科技专项重点社会发展项目(2015C03012)

摘　　要：以国家二级濒危种蛛网萼为研究材料,通过未成熟种子离体培养"、芽生芽"离体繁殖、秋水仙素诱导、生根等组织培养技术开展物种的保护与繁殖研究。结果发现:蛛网萼未成熟种子培养在木本植物用培养基(woody plant medium, WPM)+400 mg/L谷氨酰胺+500 mg/L水解酪蛋白+2 000 mg/L活性炭培养基上可被诱导萌发,35 d萌发率达40%。以种子萌发的带腋芽实生苗茎段为外植体,在WPM附加1 mg/L 6-benzylaminopurine, 6-BA)和0.5 mg/Lα-萘乙酸(1-naphthylacetic acid, NAA)的培养基上培养有利于其芽的诱导与扩大繁殖;在附加200 mg/L秋水仙素的液体MS培养基上培养,可诱导出混倍体的芽。再生芽在WPM+0.5 mg/L吲哚丁酸(indole butyric acid, IBA)培养基上进行生根并获得完整植株,部分植株野外回归至浙江凤阳山-百山祖国家级自然保护区中。本研究为解决蛛网萼种子萌发率差、植株生长缓慢、种群增强困难等问题提供了新途径。Platycrater arguta, which is one of the Class Ⅱ national endangered species in China, was selected to conduct protective and reproductive study by tissue culture techniques including in vitro culture of immature seeds, in vitro propagation of shoot, colchicine induction, and taking root. When the immature seeds were cultured on the woody plant medium (WPM)+400 mg/L glutamine+500 mg/L casein hydrolysate+2 000 mg/L activated carbon, the germination rate of P. arguta could reach 40% after 35 d. As explants, stem segments of seedlings that germinated from immature seeds were cultured on WPM with different concentrations of 6-benzylaminopurine (6-BA) and 1-naphthylacetic acid (NAA), and MS mediums with different concentrations of colchicine, respectively. The results showed that the WPM with 1 mg/L 6-BA and 0.5 mg/L NAA was best for induction and growth of regeneration buds, and the MS medium with 200 mg / L colchicine could induce mixoploids. Finally, the regeneration buds were transplanted on a WPM supplemented with 0.5 g / mL indole butyric acid (IBA) to induce roots. Some of the plants have been returned to Fengyangshan-Baishanzu National Nature Reserve, Zhejiang. This study provides a new approach to solve the problems such as the poor seed germination rate of mature seed, slow plant growth and regeneration difficulty of P. arguta.

关 键 词：蛛网萼 未成熟种子 离体繁殖 多倍体诱导 野外回归 

分 类 号：S688.9[农业科学—观赏园艺]