MeJA对低温胁迫下冬小麦抗寒生理及关键基因表达量的影响  

Effect of MeJA on the Cold Tolerance Physiology and Key Gene Expression in Winter Wheat under Low Temperature Stress

作　　者：赵虎 樊晓培 罗力力 张瑞 苍晶[1] 徐庆华[1] 于晶[1] 张达[1] ZHAO Hu;FAN Xiaopei;LUO Lili;ZHANG Rui;CANG Jing;XU Qinghua;YU Jing;ZHANG Da(College of Life Science, Notheast Agriculture Universty, Harbin, Heilongjiang 150030 , China)

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《麦类作物学报》2019年第4期407-414,共8页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(31701348;31601236;31471423);黑龙江省自然科学基金项目(C201418)

摘　　要：植物激素作为一种信号分子,在植物响应低温胁迫中起重要作用。本研究以东农冬麦1号为材料,在田间三叶期用0.5、1.0和2.0 mmol·L^(-1) MeJA喷施小麦叶片,在大田自然降温条件下,探讨外源MeJA对其抗寒生理及 TaPDF1.2、 TaCOI1、 TaMYC2基因表达量的影响。结果表明,MeJA处理降低了低温胁迫下东农冬麦1号叶片及分蘖节的相对电导率和MDA含量,提高了脯氨酸、可溶性糖和可溶性蛋白的积累,提高了抗氧化酶SOD、POD和CAT活性;在不同处理浓度中,1.0 mmol·L^(-1) MeJA效果最好,处理后小麦返青率达91.5%;-10℃时,叶片和分蘖节内积累的保护性物质最多,保护性酶活性最强。东农冬麦1号分蘖节比叶片具有更强的抗寒性。此外,1.0 mmol·L^(-1)外源MeJA处理显著提高了东农冬麦1号叶片及分蘖节中 TaPDF1.2、 TaCOI1、 TaMYC2基因的表达量。Phytohormone,as a signaling molecule,plays an important role in plants response to low temperature stress.Dongnong Dongmai 1 was used as experimental material in this study. To investigate the effect of exogenous MeJA on the cold tolerance physiology and the expression of TaPDF1.2, TaCOI1 and TaMYC2 genes in wheat,the leaves were sprayed with 0.5,1.0,and 2.0 mmol·L^-1 MeJA at the trifoliate stage,and leaves and tillering node were collected at 5 ℃,0 ℃,-10 ℃,and-25 ℃ under natural cooling conditions in field. The results showed that MeJA treatment reduced the relative electrical conductivity and MDA content of leaves and tillering node of Dongnong Dongmai 1 under low temperature stress,increased the accumulation of proline,soluble sugar and soluble protein,and increased the activities of antioxidant enzymes SOD,POD and CAT.Among different treatment concentrations,1.0 mmol·L^-1 MeJA performed best and the overwinter survival rate was 91.5%. At-10 ℃,the accumulation of protective substances in the leaves and tillering node was the highest,and the protective enzyme activity was the strongest. Compared to leaves, tillering node of Dongnong Dongmai 1 are more resistant to cold. Treatment with 1.0 mmol·L^-1 exogenous MeJA significantly increased the expression of TaPDF1.2, TaCOI1,and TaMYC2 genes in leaves and tillering node of Dongnong Dongmai 1 under low temperature stress.

关 键 词：冬小麦 低温胁迫 JA 抗寒生理 基因表达量 

分 类 号：S512.1[农业科学—作物学] S330