杏仁中央核损毁对缺钠大鼠钠欲行为启动和表达的影响  

Effects of CeA lesions on the initiation and expression of sodium appetite in sodium-deficient rats

作　　者：赵志欣 廖莹莹 范媛媛[3] 蒋恩社[1] ZHAO Zhi-xin;LIAO Ying-ying;FAN Yuan-yuan;JIANG En-she(Institute of Nursing and Health, Henan University, Kaifeng 475004;College of Nursing, Inner Mongolia Medical University,Hohhot 010050;College of Life Science, Henan University, Kaifeng 475004, China)

机构地区：[1]河南大学护理与健康研究所,开封475004 [2]内蒙古医科大学护理学院,呼和浩特010050 [3]河南大学生命科学学院,开封475004

出　　处：《中国应用生理学杂志》2019年第1期13-18,共6页Chinese Journal of Applied Physiology

基　　金：河南省高等学校重点科研项目计划(19A180013)

摘　　要：目的:探讨杏仁中央核(CeA)损毁对缺钠大鼠钠欲行为启动和表达的影响。方法:将18只成年雄性SD大鼠随机分为3组(n=6):双侧Ce A损毁组、假损毁组和不损毁组,手术恢复后给予大鼠14 d低钠饲料摄食以建立缺钠大鼠模型,运用单笼双瓶选择测试方法观察缺钠大鼠在24 h内5个不同时间段对0.3 mol/L NaCl和自由饮水的摄入情况。应用免疫荧光化学染色方法观察杏仁中央核损毁与否对缺钠或正常大鼠孤束核内醛固酮敏感神经元活动的影响。结果:低钠饮食14 d后,大鼠对0.3 mol/L NaCl 24 h内饮用量和偏爱率比低钠饮食前明显增加(P<0.01);杏仁中央核损毁后缺钠大鼠对0.3 mol/L Na Cl溶液的摄入量和偏爱率显著下降(P<0.01)。杏仁中央核损毁对低钠饮食诱发的大鼠孤束核内醛固酮敏感神经元活动增加没有影响。结论:低钠饮食诱导大鼠钠欲行为表达增加;杏仁中央核损毁压抑缺钠大鼠钠欲行为的表达,而对缺钠大鼠的钠欲行为的启动没有影响。Objective: To investigate the effects of central nucleus of amygdala (CeA) lesion on the initiation and expression of sodium appetite in sodium-deficient rats. Methods: Three groups of SD rats ( n =6 in each group) were treated with bilateral CeA lesion, sham lesion or no lesion. After the recovery, the rats were fed with low-sodium diets for 14 days to establish a sodium-deficient rat model. The double-bottle selection in single cage test was used to observe the intake of 0.3 mol/L NaCl and DW in 5 timepoint with 24 hours in sodium-deficient rats. Immunofluorescence staining of aldosterone-sensitive neurons in the nucleus tractus solitarii (NTS)was used to investigate the effect of CeA lesion or not on the activity of aldosterone-sensitive neurons in rats with or without sodium deficiency. Results: After fed with low-sodium diet for14 days, the volume and preference rate of 0.3 mol/L NaCl intake of the rats within 24 h were significantly increased compared with those before low-sodium diet ( P <0.01). The intake volume and the preference rate of 0.3 mol/L NaCl in CeA lesion rats were significantly decreased than those in CeA sham lesion rats and normal rats in the sodium-deficient condition ( P <0.01). The CeA lesion had no effects on the activity of aldosterone-sensitive neurons in NTS in rats with low-sodium diet. Conclusion: Low-sodium diet induces an increase in the expression of sodium appetite in rats. CeA lesions inhibit the behavioral expression of sodium appetite in sodium-deficient rats but have no effects on the initiation of sodium appetite in rats with sodium-deficient rats.

关 键 词：大鼠 钠欲 杏仁中央核损毁 HSD2神经元 

分 类 号：R338.3[医药卫生—人体生理学]