深海真菌Diaporthe phaseolorum FS431次级代谢产物的分离鉴定  

Isolation and identification of secondary metabolites from a deep-sea-derived fungus Diaporthe phaseolorum FS431

作　　者：郭珩 刘洪新[2] 陈玉婵[2] 李赛妮 李浩华[2] 陈闪冲 章卫民[2] 高晓霞 GUO Heng;LIU Hongxin;CHEN Yuchan;LI Saini;LI Haohua;CHEN Shanchong;ZHANG Weimin;GAO Xiaoxia(School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;School of Biosciences and Biopharmaceutics,Guangdong Pharmaceutical University,Guangzhou 510006,China;State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology,Guangzhou 510070,China)

机构地区：[1]广东药科大学药学院,广东广州510006 [2]广东省微生物研究所/省部共建华南应用微生物国家重点实验室/广东省菌种保藏与应用重点实验室/广东省微生物应用新技术公共实验室,广东广州510070 [3]广东药科大学生命科学与生物制药学院,广东广州510006

出　　处：《广东药科大学学报》2019年第2期180-185,共6页Journal of Guangdong Pharmaceutical University

基　　金：广州市科技计划重点项目(21607020018);广东省自然科学基金研究团队项目(2016A030312014);广东省海洋经济创新发展区域示范专项项目(GD2012-D01-002)

摘　　要：目的研究深海真菌Diaporthe phaseolorum FS431的次级代谢产物。方法采用正、反相硅胶柱层析色谱法、Sephadex LH-20凝胶柱色谱法和高效液相色谱法等多种色谱方法对菌株FS431的液体发酵产物进行分离纯化,并通过NMR、MS等波谱技术鉴定化合物结构。结果从发酵物中分离得到9个化合物,分别鉴定为:nectriapyrone(1)、pestalotiopyrone B(2)、nectriapyrone B(3)、(R)-甲羟戊酸内酯(4)、3,5-dihydroxy-γ-caprolactone(5)、cytochalasin H(6)、cytochalasin J(7)、脱落酸(8)和(1R,2R,4R)-trihydroxy-p-menthane(9)。结论化合物2、4、5和8首次从Diaporthe属真菌中分离得到。Objective To study the secondary metabolites from the deep-sea-fungus Diaporthe phaseolorum FS431.Methods The compounds were isolated and purified from the deep-sea-derived fungus Diaporthe phaseolorum FS431 by positive and reverse phase silica gel column chromatography,Sephadex LH-20 column chromatography and high-performance liquid chromatography (HPLC).The structures of compounds were identified by NMR,MS and other spectroscopic data.Results Nine compounds were isolated from the culture of the strain FS431 and identified as nectriapyrone (1),pestalotiopyrone B (2),nectriapyrone B (3),(R)-mevalonolactone (4),3,5-dihydroxy-γ-caprolactone (5),cytochalasin H (6),cytochalasin J (7),abscisic acid (8) and (1R,2R,4R)-trihydroxy- p -menthane (9).Conclusion Compounds 2,4,5,and 8 were isolated from the genus Diaporthe for the first time.

关 键 词：深海真菌 DIAPORTHE phaseolorum 次级代谢产物 分离纯化 

分 类 号：R282.2[医药卫生—中药学]