CD4^+CD25^+CD127^-调节性T细胞在小儿EB病毒相关噬血细胞综合征发病作用中的研究  

Experimental study on the role of CD4^+CD25^+CD127^- regulatory T cell in the pathogenesis of Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis in Children

作　　者：史红鱼 王晓欢 郝国平 朱镭 赵志华 郭慧敏 张晋丽 程艳丽 陆海燕 王静 王丽 索涛莉 张静 侯朝辉 SHI Hongyu;WANG Xiaohuan;HAO Guoping;ZHU Lei;ZHAOZhihua;GUO Huimin;ZHANG Jinli(The Children′s Hospital of Shanxi,Taiyuan 030012,China)

机构地区：[1]山西省儿童医院山西省妇幼保健院,山西太原030013

出　　处：《临床医药实践》2019年第5期333-337,共5页Proceeding of Clinical Medicine

基　　金：山西省儿童医院;山西省妇幼保健院科研项目(项目编号:201544)

摘　　要：目的:观察EB病毒(EBV)相关噬血细胞淋巴组织细胞增生症(EBV-HLH)患儿外周血CD4^+CD25^+CD127^-调节性T细胞(CD4^+CD25^+CD127^-Treg)亚群的含量,对比EBV-HLH患儿与同期来院体检的健康儿童的不同,了解EBV-HLH患儿的免疫状态,以探讨CD4^+CD25^+CD127^-Treg细胞在小儿EBV-HLH免疫中的意义。方法:建立流式细胞术检测外周血CD4^+CD25^+CD127^-Treg细胞的含量,检测30例EBV-HLH初诊患儿及30例同期来院体检的健康儿童的外周血CD4^+CD25^+Treg细胞数量。结果:EBV-HLH初诊组与正常对照组外周血CD4^+CD25^+CD127^-占CD4^+T细胞的比例分别为(3.65±1.12)%和(6.90±1.16)%,EBV-HLH初诊患儿组低于正常对照组,差异有统计学意义(P<0.05)。结论:EBV相关HLH患儿外周血中CD4^+CD25^+CD127^-Treg数量减低,表明EBV-HLH患儿免疫功能处于失衡状态。Treg细胞可能在EBV相关HLH的发生、发展中起一定作用,参与细胞免疫的调节可能是Treg细胞在EBV相关HLH免疫中的一个环节。Objective:This study was purposed to investigate the changes of CD4 ^+CD25 ^+regulatory T cells in peripheral blood of children with Epstein- Barr virus–associated hemophagocytic lymphohistiocytosis,the function of immune system and the possible roles of the CD4 ^+CD25^+regulatory T cells in immunity of Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis.Methods:The number of CD4 ^+CD25 ^+regulatory T cells were detected by flowcytometry in the peripheral blood of 30 Epstein- Barr virus-associated hemophagocytic lymphohistiocytosis in new diagnosis,and were compared with that of 30 normal children.Results:The results indicated that the mean proportion of CD4^+CD25^+ CD127^- in peripheral blood in newly diagnosed patients and normal children were (3.65±1.12)% and (6.90±1.16)% respectively,the mean proportions of CD4^+CD25^+ CD127^- in newly diagnosed patients were lower than that in normal control.Conclusion:The decrease of regulatory Tcells in pediatric patients with Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis indicate that the function of Treg cells may be in imbalance.Treg cells may play a role in occurrence and development of Epstein-Barr virus- associated hemophagocytic lymphohistiocytosis.

关 键 词：CD4 ^+CD25^+CD127^-调节性T细胞 小儿 EB病毒相关噬血细胞综合征 

分 类 号：R725.1[医药卫生—儿科]