玉米o2、o16和wx基因不同组合类型近等基因系品质分析  

Nutritious Quality Analysis of Near-isogenic Lines with Different Pyramiding Types of o2, o16 and wx Genes in Maize

作　　者：王伟 王明春[2] 任洪[2] 张开武[2] 赵德刚 杨文鹏 WANG Wei;WANG Mingchun;REN Hong;ZHANG Kaiwu;ZHAO Degang;YANG Wenpeng(The State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering,The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in MountainousRegion (Ministry of Education)/Guizhou Key Lab of Agro-Bioengineering,Institute ofAgro-Bioengineering and College of Life Sciences,Guizhou University,Guiyang 550025,China;Guizhou Institute of Upland Food Crops,Guizhou Academy ofAgricultural Sciences,Guiyang 550006,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院贵州省农业生物工程重点实验室/山地植物资源保护与种质创新省部共建教育部重点实验室绿色农药与农业生物工程国家重点实验室培育基地,贵阳550025 [2]贵州省农业科学院旱粮研究所,贵阳550006

出　　处：《种子》2019年第4期31-35,共5页Seed

基　　金：国家现代农业技术产业体系(CARS-02-082);贵州省高层次创新型人才培养对象项目(20154017;黔科合[2016]4003);贵州省科技计划项目(黔科合NY字[2014]3020号;黔科合支撑[2019]2299;黔科合重大专项字[2013]6022);贵州省自然科学基金重点项目(黔科合基础[2019]1451号);贵州省特色旱地粮食作物种质资源创新平台建设(黔科中引地[2018]4003);贵州省科研机构服务企业行动计划项目(黔科合服企[2018]4005);贵州省农业科学院专项(黔农科院院专项[2014]008号)

摘　　要：为评价o2、o16和wx基因不同组合类型近等基因系的品质,以玉米o2、o16和wx基因不同组合类型近等基因系BC 6 F 4家系及其轮回亲本为试材,利用近红外分析仪和氨基酸全自动分析仪对籽粒油分、淀粉、蛋白质和氨基酸含量进行分析。结果表明,与轮回亲本CML 539相比,o2o16wx-NIL、o2o16-NIL、o2wx-NIL和o2-NIL籽粒油分含量分别提高了11.37%、8.86%、17.53%和11.95%。o2wx-NIL和o2-NIL籽粒淀粉含量分别降低了0.71%和1.00%。o16wx-NIL和wx-NIL蛋白质含量分别提高了9.91%和10.22%,o2wx-NIL、o16wx-NIL和o16-NIL蛋白质含量分别降低了6.03%、6.05%和5.87%。o2o16wx-NIL,o2o16-NIL,o2wx-NIL,o16wx-NIL,o2-NIL、wx-NIL和o16-NIL氨基酸含量均发生不同程度变化,其中,Lys分别增加了111.22%、109.53%、91.22%、34.40%、79.93%、29.69%和70.11%。可见,o2、o16和wx基因不同组合类型近等基因系是品质基因遗传研究和育种应用的重要材料。In order to evaluate the nutritional quality of the near-isogenic lines(NILs)of the different combination types of o2,o16 and wx genes,the seed oil,starch,protein and amino acid contents were measured by the nearinfrared and automatic amino acid analyzers,using the BC 6 F 4 families of those NILs and their recurrent parent as materials.The results showed that,compared with the recurrent parent CML 539,the seed oil contents of o2o16wx-NIL,o2o16-NIL,o2wx-NIL and o2-NIL were increased by 11.37%,8.86%,17.53%and 11.95%,respectively.The seed starch contents of o2wx-NIL and o2-NIL were decreased by 0.71%and 1.00%,respectively.The seed protein contents of o16wx-NIL and wx-NIL were increased by 9.91%and 10.22%,while those of o2wx-NIL,o2-NIL and o16-NIL were decreased by 6.03%,6.05%and 5.87%,respectively.The seed lysine contents of o2o16wx-NIL,o2o16-NIL,o2wx-NIL,o16wx-NIL,o2-NIL,wx-NIL and o16-NIL were increased by 111.22%,109.53%,91.22%,34.40%,79.93%,29.69%and 70.11%,respectively.It can be seen that the near isogenic lines of different combinations of o2,o16 and wx genes are important materials for genetic research and breeding application of quality genes.

关 键 词：玉米 OPAQUE-2 opaque-16 WAXY 近等基因系 品质分析 

分 类 号：S513[农业科学—作物学]