石墨烯纳米载药体系的制备及对人口腔鳞癌细胞杀伤效果  

Preparation of Graphene Nano Drug-Loading System and Its Killing Effect on Human Oral Squamous Cell Carcinoma Cells

作　　者：张雅静 季鹏 韩德明[1] 赵丽辉[1] 徐亚娟[2] 刘文书[3] ZHANG Yajing;JI Peng;HAN Deming;ZHAO Lihui;XU Yajuan;LIU Wenshu(College of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022,China;Jilin Cancer Hospital,Changchun 130012,China;The First Hospital of Jilin University,Changchun 130021,China)

机构地区：[1]长春理工大学生命科学技术学院,长春130022 [2]吉林省肿瘤医院,长春130012 [3]吉林大学第一医院,长春130021

出　　处：《应用化学》2019年第5期564-570,共7页Chinese Journal of Applied Chemistry

基　　金：吉林省科技厅(JJKH20170604KJ)项目资助~~

摘　　要：利用化学氧化还原法制备了氧化石墨烯,进一步超声破碎剥离,得到纳米氧化石墨烯,并对其进行聚乙二醇(PEG)的功能化修饰后载药顺铂。采用扫描电子显微镜(SEM)、紫外-可见吸收光谱(UV-Vis)、傅立叶变换红外光谱(FTIR)对石墨烯纳米载药体系进行表征,细胞存活率实验(MTT)法检验石墨烯纳米载药体系对人口腔鳞癌(KB)细胞的杀伤作用。结果表明,石墨烯纳米载药体系对顺铂的负载率为42. 4%,聚乙二醇修饰后可以降低纳米氧化石墨烯的细胞毒性并提高生物相容性,对KB细胞具有双重的杀伤作用,为纳米氧化石墨烯在肿瘤治疗的临床应用提供了理论依据。Graphene oxide was synthesized by chemical redox method.The graphene oxide nanoparticles wereprepared by ultrasonic fragmentation and peeling,and functionalized by polyethylene glycol(PEG).Themodified graphene oxide was loaded with cisplatin.The graphene nano-drug delivery system was characterizedby scanning electron microscopy(SEM),ultraviolet-visible absorption spectroscopy(UV-Vis)and Fouriertransform infrared spectroscopy(FTIR).The killing effect of graphene nano-drug delivery system on oralsquamous cell carcinoma human oral squamous cell carcinoma(KB)cells was examined by cell survival rateexperiment(MTT)method.The results demonstrate that the loading rate of graphene nano-drug-loading systemis 42.4%.Polyethylene glycol modified nano graphene oxide can reduce the cytotoxicity of nano grapheneoxide and improve its biocompatibility.It has dual killing effect on KB cells,which provides a theoreticalbasis for the clinical application of nano graphene oxide in cancer treatment.

关 键 词：纳米氧化石墨烯 顺铂 细胞存活率实验 人口腔鳞癌细胞 细胞毒性 

分 类 号：O611.4[理学—无机化学]