白羽王鸽卵清蛋白关联蛋白Y基因(OVALY)克隆与生物信息学分析  

Molecular cloning and bioinformatics analysis of white king pigeon (Columba livia) ovalbumin-related protein Y gene

作　　者：徐小钦[1,2,3] 杜雪 李国勤[1,4] 张龙[2] 陶争荣 卢立志 XU Xiaoqin;DU Xue;LI Guoqin;ZHANG Long;TAO Zhengrong;LU Lizhi(Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021 , China;Institute of Ecology, China West Normal University, Nanchong 637000 , China;College of Animal Sciences & Technology, Nanjing Agricultural University, Nanjing 210095 , China;Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural Affairs, Hangzhou 310021 , China)

机构地区：[1]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 [2]西华师范大学生态研究院,四川南充637000 [3]南京农业大学动物科技学院,江苏南京210095 [4]农业农村部农产品信息溯源重点实验室,浙江杭州310021

出　　处：《浙江农业学报》2019年第5期704-708,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省农业(畜禽)新品种选育重大科技专项(2016C02054-16);浙江省农业科学院地方科技合作项目(WZ2017002)

摘　　要：旨在获得白羽王鸽(Columba livia)卵清蛋白关联蛋白Y基因(OVALY)的cDNA序列,并进行生物信息分析。研究结果显示:OVALY基因cDNA全长为2 068 bp,其中5′非编码区序列180 bp,3′非编码区序列720 bp,开放阅读框1 164 bp,编码的蛋白分子量约44.19 ku,含氨基酸残基388个(GenBank∶KX230792);与NCBI数据库中朱鹮(Nipponianippon)OVALY基因序列同源性最高(81%),与白尾鹰(Haliaeetusalbicilla)和沙鸡(Pteroclesgutturalis)OVALY基因序列的同源性分别为80%和79%。经潜在糖基化位点和磷酸化位点预测分析发现,OVALY蛋白结构中潜在糖基化位点和磷酸化位点分别为4个和22个;经CDD(conserved domain database)数据库分析发现,OVALY蛋白具有丝氨酸蛋白酶抑制剂家族的反应中心区域,属于丝氨酸蛋白酶抑制剂超家族。The experiment was conducted to clone the whole cDNA of OVALY gene in Columba livia and analyze the bioinformatic information of the cDNA sequence.The nucleotide sequence of a full-length 2 068 bp cDNA for OVALY was obtained, containing 5′UTR (180 bp), CDS (1 164 bp) and 3′UTR (720 bp), and coding 388 amino acids (44.19 ku, GenBank accession number: KX230792). The OVALY cDNA sequence of white king pigeon was most similar to Nipponianippon , and the homology was 81%. This sequence was also similar to Haliaeetusalbicilla and Pteroclesgutturalis , and the homologies were 80% and 79%, respectively. 4 N-glycosylated sites and 22 phosphorylation sites were detected in these amino acids. Conserved domain analysis of OVALY protein showed this protein had a conserved domain for serpin superfamily.

关 键 词：白羽王鸽 卵清蛋白关联蛋白Y(OVALY) RACE 

分 类 号：S813.3[农业科学—畜牧学]