子宫腺肌病内膜细胞中HAS-2和CD44的表达及雌激素对其影响  

The expression of HAS-2 and CD44 in endometrial cells of women with adenomyosis and the effect of estrogen on the expression of HAS-2 and CD44

作　　者：林斯丽 段华[1] LIN Sili;DUAN Hua(Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing,100006)

机构地区：[1]首都医科大学附属北京妇产医院,100006

出　　处：《中国计划生育学杂志》2019年第5期556-559,共4页Chinese Journal of Family Planning

基　　金：国家自然科学基金[81571412];北京市医院管理局临床医学发展专项"扬帆计划"[ZYLX201406];首都医科大学基础-临床科研合作基金[16JL44]

摘　　要：目的:探索子宫腺肌病(ADS)在位子宫内膜细胞中透明质酸合成酶(HAS)基因优势亚型和透明质酸受体CD44的表达及雌激素调节作用。方法:选择2017年10月—2018年9月在本院手术治疗的ADS患者21例为ADS组,19例无子宫内膜病变患者为对照组,qRT-PCR技术检测体外培养的子宫内膜细胞HAS各亚型(HAS1、HAS2、HAS3)和标准CD44 mRNA的表达及其对不同浓度雌激素作用的反应性。结果:ADS组子宫内膜细胞HAS2和CD44 mRNA的表达量(0.302±0.063、0.254±0.067)高于对照组(0.168±0.025、0.128±0.018)(P<0.0001),且两者在ADS组中表达呈正相关(r=0.797,P<0.0001);两组子宫内膜细胞HAS1和HAS3表达均无差异(P>0.05)。0.1、1.0、10.0 nmol/L 17β-雌二醇均能上调ADS组HAS2 mRNA表达量,与未用药0 nmol/L比较均有差异(P<0.05);对照组仅1.0 nmol/L 17β-雌二醇能显著上调HAS2 mRNA表达量,与未用药组比较有差异(P<0.05); 1.0、10.0 nmol/L 17β-雌二醇使ADS组CD44 mRNA表达量显著上调,与未用药组比较有差异(P<0.05);对照组仅10 nmol/L 17β-雌二醇可显著上调CD44 mRNA的表达量,与未用药组比较有差异(P<0.05)。结论:ADS子宫内膜细胞HAS2和CD44的表达异常增高且两者呈正相关,并受高浓度雌激素的正向调控。Objective: To explore the expression of HAS gene dominant subtypes and CD44 in cultured adenomyosis (ADS) eutopic endometrial cells in vitro, and to analyze the effect of estrogen on the expression of HAS-2 and CD44. Methods: From October 2017 to September 2018, 21 patients with ADS (ADS group) who underwent surgical treatment, and 19 patients without endometrial lesions were selected in control group. RT-qPCR was used to detect the expressions of HAS subtypes (HAS1, HAS2, HAS3) and standard CD44 of cultured endometrial cells, and also measured the responsiveness of HAS subtypes and standard CD44its by estrogen with different concentrations. Results: The expression levels of HAS2 and CD44 in endometrial cells of ADS group (0.302±0.063, 0.254±0.067, respectively) was significant higher than that (0.168±0.025, 0.128±0.018, respectively) of control group ( P <0.0001), and there was a positive correlation between the expression of HAS2 and CD44 in endometrial cells of ADS group ( r= 0.797, P <0.0001). There was no significant difference in the expression of HAS3 and HAS1 ( P >0.05). 17 beta-estradiol in 0.1 , 1.0, or 10.0 nmol/L could up-regulate the expression of HAS2 mRNA of cells in ADS group, which was significant different from that of cells without treated by 17 beta-estradiol ( P <0.05), while only 17 beta-estradiol in 1.0 nmol/L could up-regulate significantly the expression of HAS2 mRNA in control group ( P <0.05). 17 beta-estradiol in 1.0 and 10.0 nmol/L significantly increased the expression of CD44 mRNA in ADS group compared with that of control group ( P <0.05), while only 17 beta-estradiol in 10 nmol/L significantly increased the expression of CD44 mRNA of control group ( P <0.05). Conclusion: The expression of HAS2 and CD44 in ADS endometrial cells is abnormally increased, which is regulated by high concentration of estrogen.

关 键 词：子宫腺肌病 子宫内膜 透明质酸 透明质酸合成酶 透明质酸受体CD44 雌激素 

分 类 号：R711.71[医药卫生—妇产科学]