响应面法优化黄芪三种活性成分的闪式提取工艺  

Determination of optimal flash extraction condition for three active compounds of Astragalus membranaceus using response surface methodology

作　　者：王梦茹 雷振宏 梁华 李文倩 张莹 梁建萍[1] 赵建平 柴智 Wang Mengru;Lei Zhenhong;Liang Hua;Li Wenqian;Zhang Ying;Liang Jianping;Zhao Jianping;Chai Zhi(College of Life Sciences,Shanxi Agricultural University,Taigu 030801,China;Shanxi Zhendong Genuine Medicinal Material Development Co. Ltd.,Changzhi 047100,China;Shanxi Collaborative Innovation Center of Astragali Radix Resource Industrialization and Industrial Internationalization,Shanxi University of chinese Medicine,Jinzhong 030619,China)

机构地区：[1]山西农业大学生命科学学院,山西太谷030801 [2]山西振东道地药材开发有限公司,山西长治047100 [3]山西中医药大学黄芪资源产业化及产业国际化协同创新中心,山西晋中030619

出　　处：《山西农业大学学报（自然科学版）》2019年第3期14-22,共9页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家"十二五"科技支撑计划项目(2011BAI07B01);山西省黄芪资源产业化及产业国际化协同创新中心项目(HQXTCXZX2016-004);山西省科技攻关计划振东专项(2014ZD0501-1);山西省农业科技成果转化和推广示范项目(SXNKTG201810)

摘　　要：[目的]确定黄芪药效成分中3种主要活性成分闪式提取的最佳工艺条件。[方法]分别采用硝酸铝-亚硝酸钠法、苯酚-硫酸法、香草酫-硫酸法对黄芪黄酮、多糖、皂苷的含量进行测定,以各成分得率为指标,采用响应面法对提取工艺参数进行优化并得到回归模型。[结果]结果表明:回归模型较好地反映了黄酮、多糖、皂苷得率与提取条件的关系,最优工艺条件分别为,黄酮∶乙醇浓度85%、料液比1∶25、提取时间60s、提取电压120V;多糖:乙醇浓度2.5%、料液比1∶15、提取时间105s、提取电压115V;皂苷:乙醇浓度70%、料液比1∶30、提取时间120s、提取电压110V。[结论]此工艺条件下提取黄酮、多糖、皂苷的得率为0.663%、0.965%、1.126%,回归模型的预测值与实测值的相差均小于0.05%,3个回归方程与实际情况拟合较好,优化工艺可靠。[Objectives] The aim of current study was to determine the optimal technological conditions for the flash extraction of three main active components in Astragalus membranaceus .[Methods] The contents of flavonoids, polysaccharides, and saponins were determined by ultraviolet, aluminum nitrate-sodium nitrite method, phenol-sulfuric acid method, and vanilla-sulfuric acid method, respectively. The yield of each component was used as the indexes to optimize the extraction process parameters by response surface methodology, and the regression models were obtained.[Results] The results showed that the developed regression model precisely reflected the relationship between the yield of flavonoids, polysaccharides, and saponins and extraction conditions. The optimal technological conditions were determined as following: the higher concentration of flavonoids was obtained when ethanol concentration at 85 %, solid-liquid ratio at 1:25, extraction time at 60 s, and extraction voltage at 120 V;the highest concentration of polysaccharides was achieved when ethanol concentration at 2.5 %, solid-liquid ratio at 1 ∶15, extraction time at 105 s, and extraction voltage at 115 V;while the greatest concentration of saponin was reached when ethanol concentration at 70 %, solid-liquid ratio at 1 ∶30, extraction time at 120 s, and extraction voltage at 110 V.[Conclusion] Under the mentioned conditions, the yield of flavonoids, polysaccharides, and saponins were 0.663%, 0.965%, and 1.126%, respectively. The difference between the predicted and measured values of the regression models were less than 0.05%. The three regression equations fitted well to the actual situation and the optimized process was reliable.

关 键 词：黄芪活性成分 响应面法 闪式提取技术 

分 类 号：R284.2[医药卫生—中药学]