原位沉淀法制备CS/nHA多孔复合支架及其性能  

Preparation and Properties of CS/nHA Porous Composite Scaffold Based on In-situ Precipitation Method

作　　者：汪阮峰 颜世峰[1] 胡圳 尹静波[1] WANG Ruanfeng;YAN Shifeng*;HU Zhen;YIN Jingbo*Department of Polymer Materials(Department of Polymer Materials, School of Material Science and Engineering, Shanghai University, Shanghai 200444, China)

机构地区：[1]上海大学材料科学与工程学院高分子材料系,上海200444

出　　处：《高等学校化学学报》2019年第5期1080-1088,共9页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:51773113;51873101)资助~~

摘　　要：通过原位沉淀法和冷冻相分离技术得到含有钙磷前驱体(Ca P)的初始多孔支架,利用多孔支架表面原位生成的壳聚糖(CS)膜减缓Na OH溶液中OH-离子的渗透速率,以达到纳米羟基磷灰石(nHA)缓慢形成的目的,从而制得nHA分布均匀的CS/nHA多孔复合支架.利用扫描电镜(SEM)和万能试验机研究复合支架的结构和性能,发现nHA为针状结构,长度为80～200 nm,宽度为20～50 nm.随着nHA含量的增加,复合支架的孔隙率下降,由(93. 8±3. 3)%降至(87. 7±3. 8)%,压缩强度则逐渐提高,由(0. 5±0. 09) MPa增加至(1. 5±0. 06) MPa.当复合支架中nHA质量分数为25%时,未发现nHA团聚现象,nHA均匀地分布于CS基体中.通过红外光谱(FTIR)、X射线衍射(XRD)及X射线光电子能谱(XPS)等分析推断,nHA与CS之间可能存在配位和氢键作用.细胞实验结果表明,CS/nHA多孔复合支架具有良好的生物相容性,细胞在支架内部贴壁黏附生长. CS/nHA多孔复合支架有望在骨组织工程领域具有良好的应用前景.The initial porous scaffold containing calcium phosphate precursors(CaP) was obtained by in situ precipitation and freezing phase separation techniques. The chitosan(CS) membranes generated in situ on the outer layer of porous scaffolds were used to slow down the penetration rate of OH-ions in NaOH solution,so as to achieve the purpose of slow formation of nano-hydroxyapatite(nHA),and thus to prepare CS/nHA porous composite scaffolds with uniform distribution of nHA. The scanning electron microscopy(SEM) and universal testing machine were used to study the structure and properties of the composite scaffold. It was found that the nHA was acicular structure,with a length of 80 ― 200 nm and a width of 20 ― 50 nm. With the increase of nHA content,the porosity of the composite scaffold decreased from(93. 8 ± 3. 3)% to(87. 7 ± 3. 8)%,and the compressive strength gradually increased from(0. 5 ± 0. 09) MPa to(1. 5 ± 0. 06) MPa. No obvious agglomeration of nHA was found,and nHA was evenly distributed in the CS matrix when the content of nHA in the composite scaffold was 25%. It is concluded by infrared spectrometer(FTIR),X-ray diffractometer(XRD)and X-ray photoelectron spectrometer(XPS) analysis that there may be coordination and hydrogen bonding between nHA and CS. The results of cell experiments showed that the nHA/CS porous composite scaffold has good biocompatibility,and the cells adhered to the scaffold. It is expected that the CS/nHA porous composite scaffold has a good application prospect in the field of bone tissue engineering.

关 键 词：原位沉淀法 纳米羟基磷灰石 壳聚糖 骨组织工程 多孔复合支架 

分 类 号：O636[理学—高分子化学]