UPLC-HDMS法测定大鼠灌胃栀子柏皮片后小檗碱与栀子苷的药代动力学  

A Pharmacokinetic Study of Berberin and Gardenoside in Rats after Oral Administration of Zhi-Zi-Bai-Pi Tablet

作　　者：徐艳丽[1] 蒲艳春[1] 祝丽娣[2] 刘天娇[1] 孙向菊[1] 刘冬敏[1] 刘天富 XU Yan-li;PU Yan-chun;ZHU Li-di;LIU Tian-jiao;SUN Xiang-ju;LIU Dong-min;LIU Tian-fu(The 4th Hospital of Harbin Medical University Harbin,Harbin,Heilongjiang,150001,China;Heilongjiang agricultural reclamation vocational college,Harbin,Heilongjiang,150001,China;Harbin Taihua Pharmaceutical Co.,Ltd,Harbin,Heilongjiang,150001,China)

机构地区：[1]哈尔滨医科大学附属第四医院药学部,黑龙江哈尔滨150001 [2]黑龙江农垦职业学院,黑龙江哈尔滨150001 [3]哈尔滨泰华药业股份有限公司,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2019年第5期834-837,共4页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科研基金项目(12521338)

摘　　要：目的:通过UPLC-HDMS法测定小檗碱和栀子苷在大鼠灌胃栀子柏皮片后的药代动力学。方法:用固相萃取柱法处理血浆样本后,以正离子扫描方式,采用萃取离子监测(EIC)方式进行检测,采用Winnolin软件计算药物动力学参数。结果:小檗碱的达峰时间为1.89±0.56 h,其AUC值为793±87 ng/mL/h,药代参数显示该成分在体内浓度较低,消除较快;栀子苷的达峰时间为2.09±0.88 h,其AUC值为4687±345 ng/mL/h,药代参数显示该成分在体内表观分布容积较大,消除也较快。结论:利用高灵敏度的液质联用仪器在血中成功检测到了小檗碱及栀子苷,提示小檗碱及栀子苷为栀子柏皮片体内潜在药效作用物质。Objective:To study the pharmacokinetics of berberin and gardenoside after oral administration of Zhi-Zi-Bai-Pi tablet to rats.Methods:The plasma was disposed by solid phase extraction column and then analyzed in positive mode by EIC,and the pharmacokinetic parameters was analyzed by Winnolin software.Results:The Tmax of berberin was 1.89±0.56 h,and the AUC was 793±87 ng/mL/h which indicated that berberin was excreted rapidly and the concentration was very low;The Tmax of gardenoside was 2.09±0.88 h,and the AUC was 4687±345 ng/mL/h ng/mL/h which indicated that gardenoside was widely distributed and then excreted rapidly.Conclusions:Berberin and gardenoside was successfully detected in rat plasma,suggesting that berberine and geniposide are potential pharmacodynamic substances of Zhi-Zi-Bai-Pi tablet.

关 键 词：栀子柏皮片 小檗碱 栀子苷 UPLC/HDMS 

分 类 号：R-33[医药卫生] R285.5