不同表面灭菌对棉花茎秆内黄萎病菌分离效果的比较  

Comparison of Different Surface Disinfection Methods for the Isolation of the Pathogen Verticillum spp in Cotton Stalk

作　　者：孙艳[1] 石新建 王振辉[1] 冯丽凯[1] 赵静[1] 刘政[1] SUN Yan;SHI Xin-jian;WANG Zhen-hui;FENG Li-kai;ZHAO Jing;LIU Zheng(Institute of Plant Protection (IPP), Xinjiang Academy of Agricultural and Land Reclamation Sciences,Shihezi Xinjiang 832000,China)

机构地区：[1]新疆农垦科学院植物保护研究所,新疆石河子832000

出　　处：《新疆农业科学》2019年第1期104-110,共7页Xinjiang Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0201900);新疆兵团现代农业科技攻关与成果转化计划项目(2016AC005)~~

摘　　要：【目的】筛选一套标准的表面灭菌分离获得棉秆内黄萎病病原菌方法。【方法】研究4种抑制材料内细菌生长的PDA选择性培养基中最低抗生素含量,评价四种不同方法对材料表面灭菌(75%乙醇、5%次氯酸钠、0.1%升汞和75%乙醇+5%次氯酸钠)效果。【结果】含混合抗生素(链霉素:氯霉素:青霉素m/m,1∶1∶1)的PDA培养基抑制细菌最低浓度为500 mg/L;新鲜棉秆材料和轻度腐败棉秆表面灭菌的最佳条件为75%乙醇处理4 min,重度腐败棉秆表面灭菌条件为75%乙醇+5%NaClO处理5 min。【结论】即新鲜棉秆材料和轻度腐败棉秆材料表面灭菌的最佳条件为75%乙醇处理4 min,重度腐败棉秆表面灭菌条件为75%乙醇+5%NaClO处理5 min。选择合适的灭菌材料和时间是分离棉花茎杆内黄萎病菌的关键。【Objective】 To screen a standard method of surface sterilization separation in the cotton Verticillium wilt pathogen.【Method】 The lowest antibiotic content in PDA selective medium for inhibiting bacterial growth in four test materials was studied in this experiment, and four different methods were used to evaluate the effect of sterilization of the material surface(75% ethanol, 5% NaClO, 0.1% HgCl and 75% ethanol+5% NaClO).【Results】 The results showed that the lowest concentration of mixed antibiotic(streptomycin: penicillin: chloramphenicol m/m, 1∶1∶1) in PDA medium that could restrain bacteria growth was 500 mg/L;The best sterilization condition for the surface sterilization of fresh material or mild spoilage cotton stalk was 75% ethanol treatment for 4 min, and the surface sterilization condition of severe spoilage cotton stalk was 75% ethanol+5%NaClO for 5 min.【Conclusion】 Selection of suitable sterilizing material and time is the key to isolate cotton Verticillium wilt to a large extent.

关 键 词：棉花 黄萎病 抗生素 灭菌 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]