旋毛虫肌幼虫排泄分泌蛋白对非小细胞肺癌A549增殖的影响  

Observation of the Anti-tumor Action of Proteins Secreted by Trichinella Spiralis on A549 Lung Cancer Cells

作　　者：吴合亮 李美辰 潘靖丹 邵薪诺 李世昌 王一诺 杜娈英[1] WU Heliang;LI Meichen;PAN Jingdan(Chengde Medical University, Chengde City,Hebei Province 067000)

机构地区：[1]承德医学院,河北省承德市067000

出　　处：《医学理论与实践》2019年第9期1284-1285,1294,共3页The Journal of Medical Theory and Practice

基　　金：河北省教育厅重点项目(ZD2014049);河北省高校重点学科(冀教高[2013]4号);承德医学院重点项目(201709);国家级大学生创新训练计划项目(201704);省级大学生创新训练计划项目(201716);省级大学生创新训练计划项目(2018020)

摘　　要：目的:观察旋毛虫(T.spiralis)肌幼虫(ML)排泄分泌蛋白(ESPs)对非小细胞肺癌A549增殖的影响。方法:将A549细胞进行分组,旋毛虫肌幼虫排泄分泌蛋白为实验组,顺铂(DDP)为阳性对照组和不加处理的细胞为阴性对照组,通过MTT比色法检测ESPs和DDP对A549细胞增殖活性的影响。结果:MTT检测结果显示,随着ESPs浓度的增加及作用于A549细胞时间的延长,A549细胞的增殖活力逐渐减弱,ESPs对A549细胞具有明显的抑制作用,呈剂量—时间效应。结论:旋毛虫肌幼虫排泄分泌蛋白对A549细胞的增殖具有抑制作用。Objective:To observe the effect of ESPs on proliferation rate of nonsmall-cell lung cancer line A549.Methods:The A549 cells were divided into groups, excretory-secretory proteins(ESPs) of Trichinella spiralis muscle larva as experiment group, cis-platinum(DDP) as positive control group and untreated cells as negative control group. The effects of ESPs and DDP on the proliferation activity of A549 cells were detected by MTT colorimetry.Results:The results of MTT tests showed that with the increase of ESPs concentration and the extension of time acted on A549 cells, the proliferation activity of A549 cells weakened gradually.ESPs had obvious inhibitory effect on the A549 cells, and presented dose-time effect.Conclusion:This study suggested that Trichinella spiralis muscle larval excretory-secretory proteins could inhibit the growth of A549 cells.

关 键 词：旋毛虫 肌幼虫 排泄分泌蛋白 非小细胞肺癌 

分 类 号：R383.15[医药卫生—医学寄生虫学]