南丹牛Y-SNPs与Y-STRs遗传多样性研究  

Genetic Diversity of Y-SNPs and Y-STRs in Nandan Cattle

作　　者：李秀良[1] 张俸伟 曹艳红[1] 吴柱月[1] 党瑞华[2] 李绍波[1] 陈宏[2] 雷初朝[2] LI Xiu-liang;ZHANG Feng-wei;CAO Yan-hong;WU Zhu-yue;DANG Rui-hua;LI Shao-bo;CHEN Hong;LEI Chu-zhao(Animal HusbandryInstitute of Guangxi Zhuang Autonomous Region,Nanning 530001;College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100)

机构地区：[1]广西壮族自治区畜牧研究所,南宁530001 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国牛业科学》2019年第2期26-28,共3页China Cattle Science

基　　金：国家肉牛牦牛产业技术体系项目(CARS-37);利用广西黄牛杂交培育优质雪花牛肉示范与推广项目(桂渔牧科201452035);广西良种肉牛繁育关键技术与产业化应用示范项目(桂科AA17204028).

摘　　要：[目的]分析南丹牛的Y染色体遗传多样性及父系起源。[方法]用PCR扩增、测序及生物信息学方法进行分析。[结果]通过对25头南丹牛的Y-SNPs和Y-STRs分析,发现南丹牛仅包含瘤牛Y3单倍型组,细分为Y3-88-156和Y3-90-156两种单倍型,单倍型频率分别为92%和8%,南丹牛的Y染色体单倍型多样度为0.1533±0.0915。[结论]南丹牛是瘤牛父系起源,其遗传基础很稳定。[Objective]The aim of this paper was to analyze Y chromosome genetic diversity and the paternal origin of Nandan cattle.[Methods] Polymerase chain reaction amplification, sequencing and bioinformatics were used for this study.[Results] Based on the analysis of Y-SNPs and Y-STRs markers of 25 Nandan cattle male samples, the results indicated that the Nandan cattle belonged to only zebu Y3 haplogroup,was divided into two haplotypes: Y3-88-156 and Y3-90-156,and their haplotype frequencies were 92% and 8%, respectively.The Y chromosome haplotypediversity was 0.1533 ± 0.0915 in Nandan cattle.[Conclusion] Nandan cattle belong to the zebu paternal origin with low paternal genetic diversity, indicating a very stable genetic basis.

关 键 词：南丹牛 Y-SNPS Y-STRS 遗传多样性 父系起源 

分 类 号：S823.81[农业科学—畜牧学]