鲍曼不动杆菌CRISPR系统筛查及对靶基因的调控作用  

Screening of CRISPR system in Acinetobacter baumannii and analysis of its regulation on target genes

作　　者：冯欢欢 李金月 王宇航[1] 李梦影 郑文浩 李端阳 傅夏燕 何新龙 李国才[1] FENG Huanhuan;LI Jinyue;WANG Yuhang;LI Mengying;ZHENG Wenhao;LI Duanyang;FU Xiayan;HE Xinlong;LI Guocai(Jiangsu Key Laboratory of Noncoding RNA/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses/College of Medical,Yangzhou University,Yangzhou 225009, China)

机构地区：[1]扬州大学医学院/江苏省非编码RNA重点实验室/江苏省动物重要疫病与人兽共患病防控协同创新中心

出　　处：《扬州大学学报（农业与生命科学版）》2019年第2期54-57,64,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(81471906);扬州大学科技创新团队培养对象资助项目(2016-09)

摘　　要：对89株临床分离株采用PCR产物克隆测序和成簇规律间隔短回文重复序列(CRISPR)系统生物信息学分析等方法研究鲍曼不动杆菌CRISPR系统对靶基因的调控作用。结果表明:2株分离株(AB43与ATCC19606)具有CRISPR系统,它们间区靶向的基因有IV型分泌蛋白Virb5、带状黏附毒素蛋白、噬菌体蛋白、DNA聚合酶等。CRISPR系统抑制Virb5、带状黏附毒素蛋白基因的编码与表达,可能对鲍曼不动杆菌的生物膜形成具有一定调控作用。PCR cloning, sequencing and CRISPR analysis were performed in 89 clinical isolates to explore the regulation of A?baumannii CRISPR system on its target genes. The results showed that the two strains, AB43 and ATCC19606, contained a CRISPR system respectively. The genes targeted by them included type IV secreted protein Virb5, zonular occludens toxin protein, phage protein, DNA polymerase, etc. The inhibition of CRISPR system on the expression of Virb5 and zonular occludens toxin genes indicated a certain regulation of the biofilm formation of Acinetobacter baumannii.

关 键 词：鲍曼不动杆菌 CRISPR 生物膜 

分 类 号：R378.99[医药卫生—病原生物学]