Effect of electroacupuncture on anti-Mullerian hormone expression in rats with polycystic ovarian syndrome  

电针对多囊卵巢综合征大鼠抗苗勒氏管激素水平的影响

作　　者：Zhou Jing Sun Yi Sun Jie Zhao Ji-meng Cui Yun-hua Wu Ling-xiang Wu Huan-gan Shi Yin Li Liang 周竞;孙怡;孙洁;赵继梦;崔云华;吴凌翔;吴焕淦;施茵;李亮(Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai Songjiang District Central Hospital, Shanghai 201600, China Shanghai Songjiang District Central Hospital, Shanghai 201600, China;Shanghai Research In stitute of Acupuncture and Meridian, Shanghai 200030, China;China Academy of Chinese Medical Sciences, Beijing 100700,China)

机构地区：[1]Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China [2]Shanghai Songjiang District Central Hospital, Shanghai 201600, China Shanghai Songjiang District Central Hospital, Shanghai 201600, China [3]Shanghai Research In stitute of Acupuncture and Meridian, Shanghai 200030, China [4]China Academy of Chinese Medical Sciences, Beijing 100700,China

出　　处：《Journal of Acupuncture and Tuina Science》2019年第2期80-88,共9页针灸推拿医学（英文版）

基　　金：国家重点基础研究发展计划项目(No.2015CB554500);中国中医科学院自主选题研究项目(No.ZZKF08002);上海市卫生和计划生育委员会科研课题(No.201840307);上海市中医药领军人才项目(No.ZYSNXD-RC-LJRC)。

摘　　要：Objective: To observe whether the effect of electroacupuncture (EA) on improving sex hormone disorders and follicle development is by decreasing the expression of anti-Mullerian hormone (AMH) in rats with experimental polycystic ovarian syndrome (PCOS). Methods: Forty rats were randomly divided into four groups, a normal group (NG), a model group (MG), an EA at acupoints group (EAAG), and an EA at non-acupoints group (EANAG), with 10 rats in each group. The rats in the EAAG and EANAG were intervened by EA treatment for consecutive 14 d. Zhongji (CV 3) and Guanyuan (CV 4) were selected as the acupoints in the EAAG, and the tip of the tail and 1 cm up from the tail tip were selected as the non-acupoints in the EANAG. After treatment, the histomorphological changes of the ovary, the levels of aromatase P450 (P450arom)/ testosterone and estradiol in the ovarian tissues, and the expressions of follicle stimulating hormone (FSH) and AMH were observed. Results: After treatment, compared with the MG and EANAG, the expression of AMH decreased (P<0.05), the levels of P450arom and estradiol in creased sign ifica ntly, and the level of testostero ne decreased sign ifica ntly (all P<0.01) in the EAAG. Additionally, several normal follicles were present and the number of cystically dilated follicles decreased in the EAAG. Compared with the MG and EANAG, the EAAG obviously had more follicular granulosa cells. Con elusion: EA can dow n-regulate the abn ormally in creased expressi on of AMH to improve sex hormone disorders and follicle development in PCOS rats.目的:观察电针是否通过降低抗苗勒氏管激素(AMH)的表达实现对多囊卵巢综合征(PCOS)大鼠性激素紊乱和卵泡发育障碍的改善作用。方法:将40只大鼠随机分为正常组、模型组、电针经穴组和电针非经穴组,每组10只。电针经穴组和电针非经穴组大鼠连续治疗14 do电针经穴组大鼠取中极和关元;电针非经穴组取尾尖和尾尖上1 cm处。观察治疗后卵巢组织形态学变化,P450芳香化酶、睾酮和雌二醇水平以及促卵泡生成素(FSH)和AMH的表达。结果:同模型组和电针非经穴组比』交,电针经穴组AMH表达降低(PV0.05), P450芳香化酶和雌二醇水平显著升高,睾酮水平显著降低(均P<0.01)o此外,电针经穴组存在数个正常卵泡,囊性扩张卵泡减少,卵泡颗粒细胞数明显多于模型组和电针非经穴组。结论:电针可下调PCOS大鼠AMH的异常过度表达,从而改善性激素紊乱和卵泡发育障碍。

关 键 词：Acupuncture Therapy ELECTROACUPUNCTURE Point  Zhongji(CV 3) Point  Guanyuan (CV 4) Anti-Mullerian Hormone Polycystic Ovary Syndrome Follicle Stimulating Hormone RATS 

分 类 号：R2-03[医药卫生—中医学]