超氧对主动脉夹层患者围术期红细胞氧化应激以及能量代谢影响的体外实验研究  

Effect of ozone on oxidative stress and energy metabolism change of blood from aortic dissection patients in vitro

作　　者：邓丽[1] 杨慧[1] 刘宏宇[1] 苏晶晶[1] 胡光 迟超[1] DENG Li;YANG Hui;LIU Hongyu;SU Jingjing;HU Guang;CHI Chao(Department of Cardiovascular Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, 150006, P.R.China)

机构地区：[1]哈尔滨医科大学附属第一医院心脏外科,哈尔滨150006

出　　处：《中国胸心血管外科临床杂志》2019年第5期465-469,共5页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：黑龙江省卫生厅资助项目(2018-382);国家临床重点专科建设项目

摘　　要：目的观察不同浓度超氧对主动脉夹层(aortic dissection,AD)患者围术期体外血液样本中红细胞氧化应激以及能量代谢的影响,为临床应用超氧自体血疗法(ozonated autohemotherapy,ozone-AHT)提供实验基础。方法收集哈尔滨医科大学附属第一医院2016年3～8月20例AD患者[男16例、女4例,平均年龄(48.51±10.21)岁]手术前后的血液标本进行不同浓度(0μg/ml,40μg/ml,60μg/ml,80μg/ml,160μg/ml)的超氧(ozone)预处理。酶联免疫吸附剂测定法(ELISA)测定氧化/抗氧化指标[丙二醛(MDA)、超氧化物歧化酶(SOD)]以及能量代谢指标[Na^+-K^+-ATP、 2,3二磷酸甘油酸(2,3-DPG)]。结果在对照组(0μg/ml)中,术后MDA含量比术前含量显著增加(P<0.05),术后SOD、Na^+-K^+-ATP、2,3-DPG较术前组显著减少(P<0.05)。40μg/ml、60μg/ml、80μg/ml组术后MDA含量比术前组增加(P>0.05),SOD、Na^+-K^+-ATP、2,3-DPG较术前组减少(P>0.05);术前三个浓度组组间(40μg/ml、60μg/ml、80μg/ml)与术后三个浓度组组间(40μg/ml、60μg/ml、80μg/ml)MDA、SOD、Na^+-K^+-ATP、2,3-DPG数值变化差异无统计学意义(P>0.05);160μg/ml浓度组与其它所有浓度组相比,术前、术后MDA含量均增加,术前、术后SOD、Na^+-K^+-ATP、2,3-DPG均减少,差异有统计学意义(P<0.05)。结论 40～80μg/ml的ozone可以提高AD患者血液样本中红细胞膜抗氧化能力、降低氧化应激反应、提高红细胞膜的能量代谢,因此该浓度范围的ozone应用于AD围术期ozone-AHT是安全可行的。Objective To investigate the effect of ozone on oxidative stress and energy metabolism change of blood from aortic dissection(AD) patients for providing preliminary evidence of application of ozonated autohemotherapy(ozone-AHT) in AD patients. Methods Twenty AD patients(16 males and 4 females with a mean age of 48.51±10.21 years) were consecutively included in the First Affiliated Hospital of Harbin Medical University from March 2016 to August 2016, and blood samples were collected from all participants and ozonized in vitro at different ozone concentrations(0 μg/ml, 40 μg/ml, 60 μg/ml, 80 μg/ml, 160 μg/ml). Malondialdehyde(MDA), red blood cells(RBCs) superoxide dismutase(SOD), Na^+-K^+-ATP, 2,3-bisphosphoglyceric acid(2,3-DPG) at different ozone concentrations were evaluated by enzyme-linked immunosorbent assay(ELISA). Results In the control group(0 μg/ml),the content of postoperative MDA was significantly higher than that of preoperation(P<0.05). The contents of postoperative SOD, Na+-K+-ATP and 2,3-DPG were significantly lower than that of preoperation(P<0.05). The content of MDA at the concentrations of 40 μg/ml, 60 μg/ml, 80 μg/ml group increased after the operation(P>0.05), and the SOD,Na+-K+-ATP, 2,3-DPG decreased compared with the preoperation(P>0.05). But all the values were not statistically significant at the concentrations of 40 μg/ml, 80 μg/ml and 160 μg/ml respectively between preoperation and postoperation(P>0.05). Compared with other concentration groups, the content of preoperative and postoperative MDA increased in the ozone group(160 μg/ml), and oppositely, the contents of preoperative and postoperative SOD, Na+-K+-ATP and 2,3-DPG decreased(P<0.05). Conclusion The concentrations of 40 to 80 μg/ml of ozone can improve the antioxidant capacity of erythrocyte membrane, reduce oxidative stress in blood samples of AD patients and improve the energy metabolism of erythrocyte membranes, so the concentration range of ozone is safe and feasible for the ozone-AHT of perioperative AD.

关 键 词：主动脉夹层 超氧 氧化应激 能量代谢 

分 类 号：R654.2[医药卫生—外科学]