分子标记及RAD测序辅助选择改造两系不育系水稻粒型  

Molecular Markers and RAD Sequencing Assistant Selection for Improvement of Grain Size in Two-line Sterile Rice

作　　者：侯军亮 席杏媛 李世明 李泽桦 李刚 伏琦 李云飞 鄢阳天 杨麒生 喻涵 夏秋菊 刘宝龙[6] 李勇 张耕耘 赵山岑 倪雪梅 Hou Junliang;Xi Xingyuan;Li Shiming;Li Zehua;Li Gang;Fu Qi;Li Yunfei;Yan Yangtian;Yang Qisheng;Yu Han;Xia Qiuju;Liu Baolong;Li Yong;Zhang Gengyun;Zhao Shancen;Ni Xuemei(BGI-Shenzhen, Shenzhen,518083;BGI Institute of Applied Agriculture, Shenzhen,518083;Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining,810000;State Key Laboratory of Agricultural Genomics, Shenzhen,518083;Key Laboratory of Genomics, Ministry of Agriculture, Shenzhen,518083;Shenzhen Engineering Laboratory of Crop Molecular Design Breeding, Shenzhen,518116)

机构地区：[1]深圳华大生命科学研究院,深圳518083 [2]深圳市华大农业应用研究院,深圳518083 [3]农业基因组学国家重点实验室,深圳518083 [4]农业部农业基因组学重点实验室(深圳),深圳518083 [5]深圳作物分子设计聚合育种工程实验,深圳518116 [6]中国科学院西北高原生物研究所,西宁810000

出　　处：《分子植物育种》2019年第8期2552-2558,共7页Molecular Plant Breeding

基　　金：深圳市科技计划项目(CXZZ20150330171810060);深圳作物分子设计聚合育种工程实验室提升项目(深发改(2015)946号);国家高技术研究"863"发展计划(2014AA10A602-4);国家科技支撑计划(2015BAD02B01-7)共同资助

摘　　要：长粒型稻米具有垩白度低,直链淀粉含量高,蒸煮后坚韧蓬松的优点。为了改良水稻粒型,本研究以‘R15’为长粒型基因供体材料,‘Y58S’光温敏两系不育系作为轮回亲本进行改良,开发出了与控制粒型的qGL7紧密连锁的分子标记R072774。利用该分子标记在每一代选出含有长粒型基因的株系进行建库测序,利用RAD重测序数据进行基因组选择,选择含有长粒型基因且基因组背景最接近轮回亲本‘Y58S’的个体。从F2代开始进行回交,经过4代回交和1代自交,F2代及回交后代与‘Y58S’遗传背景平均相似率为F2(37.07%)<BC1F1(53.03%)<BC2F1(97.89%)。最终得到的新品系系‘深华香15S’总共有356 920 821 bp的片段与轮回亲本‘Y58S’一致,占基因组的95.62%。7号染色体22 482 655～29 697 621 bp区间被‘R15S’替换,使得新品系携带有长粒型基因。与亲本‘Y58S’相比,‘深华香15S’籽粒长度增加29%,长宽比增加了51.7%,成功培育出长粒型水稻不育系。本研究为快速选择、培育长粒型水稻提供了理论和实践参考。Long grain rice has the advantages of low degree of chalkiness,high content of amylose and firm and fluffy after cooking.In order to improve the rice grain shape,in this study,‘R15’was used as long-grained gene donor material and‘Y58 S’,photo-thermo-sensitive two-line sterile line,was used as a recurrent parent to develop a mo lecular marker R072774 closely linked to qGL7 controlling grain shape.Using this molecular marker,the individuals containing the long-grained gene were selected in each generation for the establishment of the genomic library,and the genome selection was performed using RAD resequencing data.The individuals containing the long-grained gene and the genome background closest to the recurrent parent‘Y58 S’were selected.Backcrossing from the F2 generation,after 4 generations of backcross and 1 generation of self-crossing,the average genetic background similarity of F2 generation and backcross generations compared with‘Y58 S’was F2(37.07%)<BC1F1(53.03%)<BC2F|(97.89%).The resulting new line'Shenhuaxiang 15S'had a total of 356 920 821 bp fragments consistent with the recurrent parent'Y58S',accounting for 95.62%of the genome.The interval between 22 482 655 bp to 29 697 621 bp of chromosome 7 was replaced by R15S,making the new line with long-grained genes.Compared with the parent'Y58S','Shenhuaxiang 15S'grain length increased by 29%,and the length-width ratio increased by 51.7%.Thus,we successfully cultivated a long-grain rice sterile line.This study could provide a theoretical and practical reference for the rapid selection and cultivation of long-grain rice.

关 键 词：分子标记辅助选择 RAD测序 qGL7 光温敏两系不育系 

分 类 号：S511[农业科学—作物学]