基于位点特异性PCR鉴别多花黄精及其混淆品  

Identification of Polygonatum cyrtonema and Its Adulterants Based on Site-specific PCR

作　　者：狐小斌 季鹏章[1] 朱新焰[1] 石亚娜[1] 李彦莹[1,2] 王家金 李玲玉[1] Hu Xiaobin;Ji Pengzhang;Zhu Xinyan;Shi Yana;Li Yanying;Wang Jiajing;Li Lingyu(Institute of Medical Plant,Yunnan Academy of Agricultural Science,Kunming,650223;College of Food Sciences and Technology, Yunnan Agricultural University, Kunming,650092)

机构地区：[1]云南省农业科学院药用植物研究所,昆明650223 [2]云南农业大学食品科技学院,昆明650092

出　　处：《分子植物育种》2019年第8期2587-2591,共5页Molecular Plant Breeding

基　　金：国家自然科学基金(31760347);道地药材国家重点实验室培育基地开放课题;云南省科技计划项目(2015-FB161; 2016RA057);云南省技术创新人才培养项目(2018HB085);云南省级环境保护专项资金项目(2014BI006);昆明市生物产业项目(2016-17)共同资助

摘　　要：为了建立快速区分多花黄精及其混淆品的鉴别方法,本研究采用位点特异性PCR方法,通过对多花黄精及其混淆品的psb A-trnH片段进行扩增。同源比对后根据其变异位点设计特异性鉴别引物,分析多花黄精及其混淆品的位点特异性差异情况。结果表明:本研究设计的特异性鉴定引物,在一个PCR反应中多花黄精能扩增出329 bp的条带,而混淆品不能扩增出条带,从而实现了正伪品的鉴别。该方法有效区别多花黄精与其混淆品品种,经多次试验,重复性较好。本研究为快速区分多花黄精及其混淆品提供了有效办法。In order to establish a rapid identification method for Polygonatum cyrtonema and its adulterants,site-specific PCR was used to amplify the psbA-trnH fragment of Polygonatum cyrtonema and its adulterants. After homology comparison, specific primers were designed according to their mutation sites, and the site specificity differences between Polygonatum cyrtonema and its adulterants were analyzed. The results showed that the specific identification primers designed in this study could amplify 329 bp bands of Polygonatum cyrtonema in a single PCR reaction, while the adulterants could not amplify the bands, thus realizing the identification of authentic and counterfeit products. This method could effectively distinguish Polygonatum cyrtonema and its adulterants. After many experiments, the method had good repeatability. This study could provide an effective method for quickly distinguishing Polygonatum cyrtonema and its adulterants.

关 键 词：多花黄精 位点特异性PCR 分子鉴定 

分 类 号：R282.5[医药卫生—中药学]