Biosynthesis of Triacylglycerol Molecules with a Tailored PUFA Profile in Industrial Microalgae  

作　　者：Yi Xin Chen Shen Yiting She Hong Chen Cong Wang Li Wei Kangsup Yoon Danxiang Han Qiang Hu Jian Xu 

机构地区：[1]Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China [2]Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, China [3]Core Laboratory, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China [4]Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266237, China [5]University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Molecular Plant》2019年第4期474-488,共15页分子植物（英文版）

基　　金：the Natural Science Foundation of China (31600059, 31425002, and 31741005), the Natural Science Foundation of Shandong, China (ZR2015CQ003 and ZR201709180185);the Biological Carbon Sequestration Program (ZDRW-ZS-2016-3) from the Chinese Academy of Sciences;the Chinese Key R&D Plan for Synthetic Biology Project.

摘　　要：The composition of polyun saturated fatty acids (PUFAs) in triacylglycerols (TAGs) is key to health ben efits and for oil applications, yet the underlying genetic mechanism remains poorly understood. In this study, by in si Iico, ex vivo, and in vivo profili ng of type-2 diacylglycerol acyltransferases (DGAT2s) in Nannochloropsis oceanica we revealed two novel PUFA-preferring enzymes that discriminate individual PUFA species in TAG assembly, with NoDGAT2J for linoleic acid (LA) and NoDGAT2K for eicosapentaenoic acid (EPA). The LA and EPA composition of TAG molecules is mediated in vivo via the functional partitioning between NoDGAT2J and 2K, both of which are localized in the chloroplast envelope. By modulating transcript abundance of the DGAT2s, an N. oceanica strain bank was created, where proportions of LA and EPA in TAG vary by 18.7-fold (between 0.21% and 3.92% dry weight) and 34.7-fold (between 0.09% and 3.12% dry weight), respective!y. These findings lay the foundation for producing designer TAG molecules with tailored health benefits or for biofuel applications in industrial microalgae and higher-plant crops.

关 键 词：MICROALGAE polyunsaturated fatty acids DIACYLGLYCEROL ACYLTRANSFERASE NANNOCHLOROPSIS oceanica 

分 类 号：Q[生物学]