云南省2010—2013年病毒性脑膜炎病例中埃可病毒30型的VP1基因特征分析  

Genetic characteristic analysis of the VP1 gene of echovirus 30 isolated from viral meningitis cases in Yunnan province, 2010-2013

作　　者：何丽芳[1] 李徽[1] 李凯[1] 赵智娴[1] 丁峥嵘[1] 田炳均[1] He Lifang;Li Hui;Li Kai;Zhao Zhixian;Ding Zhengrong;Tian Bingjun(Yunnan Center for Disease Control and Prevention, Kunming 650022, China)

机构地区：[1]云南省疾病预防控制中心,昆明650022

出　　处：《中华实验和临床病毒学杂志》2019年第2期158-162,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的对云南省2010—2013年病毒性脑膜炎(viral meningitis,VM)病例中分离到的9株埃可病毒30型(echovirus 30, E-30)VP1编码序列基因特征进行分析。方法对云南省2010—2013年VM病例中分离到的9株E-30病毒的VP1区进行基因扩增和测序;从GenBank下载E-30病毒参考株(reference)和原型株(prototype)VP1编码序列作为参考序列,用MEGA5.1软件计算9株病毒之间及它们与原型株和参考株之间的核苷酸(nucleotide,nt)和氨基酸(amino acid,aa)差异率,并构建系统进化树,进行VP1编码序列基因特征和分子流行病学分析。结果2010—2013年云南省从553例VM病例中共分离到79份肠道病毒(enterovirus,EV),其中E-30病毒9株,占11.39%(9/79),E-30的阳性检出率为1.63%(9/553)。基因特征分析表明,E-30病毒可分为A、B、C、D基因型(genotype),D基因型又分为7个亚型,本次云南的E-30病毒分离株均为D7基因亚型(sub-genotype D7), 9株病毒分布在3个不同的进化分支中。其中,5株2010年7月下旬分离到的病毒形成1个单独的进化分支,结合流行病学分析,这些病毒株可确定为引起当年昆明市周边地区一起E-30 VM暴发流行的病因;2011年的1株E-30病毒和2013年分离株与2010年云南省边境健康儿童分离株共同形成了第2个进化分支,3株E-30病毒之间亲缘较近,具有同一进化来源,以上2个分支均为云南省特有的进化分支;2011年的另外2株病毒则和2003年中国山东、江苏、浙江省VM暴发流行时分离到的病毒形成了第3个进化分支,这些病毒株可能具有同一进化来源。结论本次云南的9株E-30病毒分离株均为D7基因亚型,但9株病毒具有不同的进化来源,可见不同时间E-30病毒的同一基因亚型进化分支可在不同地区间流行。Objective In this study we analyzed the genetic characteristics of echovirus 30 (E-30) VP1 gene sequences from Yunnan province isolated from viral meningitis (VM) cases in 2010-2013. Methods RT-PCR and VP1 gene sequencing were done for 9 E-30 strains isolated from VM cases in 2010-2013. VP1 gene sequences of E-30 reference strains were downloaded from the GenBank and their nucleotide (nt) and amino acid (aa) diversities were calculated by MEGA 5.1 software, the phylogenetic tree was constructed and the genetic characteristics and molecular epidemiology were analyzed. Results In 2010-2013, 9 strains of E-30 viruses were detected from 79 VM cases caused by echoviruses, accounting for 11.39%(9/79), the overall positive rate was 1.63%(9/553). Phylogenetic analysis revealed that E-30 strains can be divided into four genotypes (genotype A, B, C and D), and genotype D can be further divided into seven sub-genotypes. Nine Yunnan VM isolates were distributed in D7 sub-genotype, and can be further clustered into 3 branches: 5 strains isolated in 2010 were clustered in branch 1, it is evident that these viruses were responsible for an aseptic meningitis outbreak in Kunming in that year;one 2011 isolate, together with 2013 isolate and one isolate from healthy children in 2010 were clustered in branch 2, these two branches were Yunnan special branches, and two 2011 isolates had the highest homology with 2003 VM outbreaks′ strains isolated from Shandong, Jiangsu, and Zhejiang, showing that these strains may have the same evolutionary sources. Conclusions Nine Yunnan VM isolates were distributed in D7 sub-genotype, and these strains have different evolutionary sources, showing that at different times E-30 viruses in the same sub-genotypes branch might prevail in different areas.

关 键 词：埃可病毒30型 VP1基因 基因特征分析 

分 类 号：R512.3[医药卫生—内科学] R440[医药卫生—临床医学]