Genome-wide analysis of odorant-binding proteins and chemosensory proteins in the sweet potato whitefly, Bemisia tabaci  

作　　者：Yang Zeng Yu-Ting Yang Qing-Jun Wu Shao-Li Wang Wen Xie You-Jun Zhang 

机构地区：[1]Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China [2]Department of Agriculture of Yangtze University, Jingzhou, Hubei Province, China

出　　处：《Insect Science》2019年第4期620-634,共15页昆虫科学（英文版）

基　　金：the National Natural Science Foundation of China (31420103919, 31672032 and 31772172);Beijing Nova Program (Z171100001117039);Science and Technology Innovation Project from the Chinese Academy of Agricultural Sciences (CAASXTCX2016017002)and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables.

摘　　要：Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) of insects are thought to play roles in olfactory recognition affecting host choice, copulation, reproduction and other behaviors. Previous descriptions of OBPs and CSPs in the whitefly Bemisia tabaci often provided no or incomplete genetic information. In this study, we present a genome-wide and transcriptome-wide investigation of the OBPs and CSPs in B. tabaci MEAMl (Middle East-Asia Minorl species). Eight OBP and 19 CSP genes were identified that covered all previous sequences. Phylogenetic analyses showed that the CSP genes had a lineage-specific expansion (BtabBCSP人 BtabBCSP3, BtabBCSPl3, BtabBCSPl 7, BtabBCSP18 and BtabBCSP 19). Expression profiling of OBPs and CSPs by transcriptome sequencing and quantitative real-time polymerase chain reaction (qPCR) revealed that expression patterns differed among developmental stages of B. tabaci MEAM 1. Five OBP genes and 11 CSP genes significantly differed between males and females;four of the 19 CSP genes were highly expressed in adults, while two were highly expressed in nymphs. The expression profiles of the OBP and CSP genes in different tissues of B. tabaci MEAM1 adults were analyzed by qPCR. Four OBP genes found in B. tabaci MEAM 1 were highly expressed in the head. Conversely, only two CSPs were enriched in the head, while the other six CSPs were specifically expressed in other tissues. Our results provide a foundation for future research on OBPs and CSPs in B. tabaci.

关 键 词：BEMISIA TABACI CSPs expression PATTERNS GENOME-WIDE identification OBPs PHYLOGENETIC 

分 类 号：Q[生物学]