足月分娩巨大儿的产妇胎盘IGF-1基因表达和启动子区CpG位点甲基化水平研究  

Study on the expression of IGF-1 gene in placenta and the methylation level of CpG locus in promoter region of full-term macrosomia

作　　者：张志革 高敏 马琳 ZHANG Zhi-ge;GAO Min;MA Lin(Department of Reproductive Medicine,Xingtai City Third Hospital,Xingtai Hebei 054000,China.)

机构地区：[1]邢台市第三医院生殖医学科,河北邢台054000

出　　处：《临床和实验医学杂志》2019年第9期945-948,共4页Journal of Clinical and Experimental Medicine

基　　金：河北省自然科学基金项目(编号:H2012406016)

摘　　要：目的研究足月分娩巨大儿的产妇胎盘胰岛素样生长因子l(IGF-1)基因表达和启动子区Cp G位点甲基化水平。方法回顾性选取邢台市第三医院收治的2017年9月至2018年6月102例正常妊娠且足月分娩的产妇和新生儿,根据新生儿出生体重进行分组,以正常体重儿为对照组(57例),以巨大儿(45例)为观察组。比较两组胎盘IGF-1基因表达水平和DNA甲基化水平等情况,并采用Pearson相关性分析胎盘IGF-1基因表达量与IGF-1甲基化水平及新生儿出生体重的关系。结果两组胎盘IGF-1基因表达水平和甲基化水平的比较,并无显著差异(P>0. 05)。经Pearson相关性分析结果显示,胎盘IGF-1 mRNA相对表达量与IGF-1甲基化水平并无明显关系(P>0. 05)。两组胎盘启动子区Cp G位点均呈现低甲基化水平。以所有新生儿出生体重的均值(4 257 g)为临界值,结果发现相比出生体重≥4 257 g组,出生体重<4 257 g组IGF-1 mRNA相对表达量明显升高(P <0. 05)。经Pearson相关性分析结果显示,胎盘IGF-1 mRNA相对表达量与新生儿出生体重存在正相关关系(r=0. 48,P <0. 01)。结论产妇胎盘IGF-1基因表达量与新生儿出生体重存在正相关关系,其表达量的高低均与处在低甲基化程度的胎盘IGF-1中的甲基化水平并无密切关系。Objective To study the expression of insulin-like growth factor-1(IGF-1)gene in placenta and the methylation level of CpG locus in promoter region of full-term macrosomia.Methods 102 normal pregnant women and newborns were selected from September 2017 to June 2018.According to the birth weight of newborns,they were divided into control group(57 cases)and observation group(45 cases).The levels of IGF-1 gene expression and DNA methylation in placenta of the two groups were compared.Pearson correlation was used to analyze the relationship between the level of IGF-1 gene expression and the level of IGF-1 methylation in placenta.Results There was no significant difference in the level of IGF-1 gene expression and methylation between the two groups(P>0.05).Pearson correlation analysis showed that there was no significant correlation between the relative expression of IGF-1 and the methylation level of IGF-1 in placenta(P>0.05).The CpG loci in the promoter region of placenta showed low methylation level in both groups.Taking the mean birth weight of all newborns(4 257 g)as the critical value.The results showed that the relative expression of IGF-1 mRNA in the group with birth weight<4 257 g was significantly higher than that in the group with birth weight(≥4 257 g)(P<0.05).Pearson correlation analysis showed that the relative expression of IGF-1 in placenta was positively correlated with the birth weight of newborns(r=0.48,P<0.01).Conclusion There is a bidirectional relationship between the expression of IGF-1 gene in placenta of parturient women and the birth weight of newborns.The expression of IGF-1 gene is not closely related to the methylation level of placenta IGF-1 with low methylation level.

关 键 词：巨大儿 胰岛素样生长因子l DNA甲基化 出生体重 胎盘 

分 类 号：R714.7[医药卫生—妇产科学]