JNK1在2型糖尿病大鼠股动脉纤维病变中的表达及中药桃仁干预研究  

JNK1 in Ⅱ diabetes rat femoral artery expression and traditional Chinese medicine in the lesions of peach kernel intervention study

作　　者：武海阔[1] 张荣荣 王军[1] 李梦虎[1] WU Haikuo;ZHANG Rongrong;WANG Jun;LI Menghu(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China)

机构地区：[1]天津中医药大学第一附属医院,天津300193

出　　处：《天津中医药》2019年第5期486-490,共5页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81473685)

摘　　要：[目的]观察2型糖尿病大鼠下肢股动脉血管中c-Jun氨基末端激酶(JNK1)的表达及中药桃仁的干预作用。[方法]雄性SD大鼠50只,随机分组为:空白对照组、模型对照组、早期干预组、桃仁高剂量组、桃仁低剂量组各10只;造模成功后,实验组予药物连续干预3周,取材进行实验检测,观察各项指标表达情况。[结果] 1)模型组JNK1的表达较空白对照组明显升高;2)中药桃仁可有效降低JNK1表达,可明显改善糖尿病大鼠股动脉病变情况,减缓纤维化进程,以早期干预组效果最为明显,桃仁高剂量组效果良好,桃仁低剂量组效果欠佳。[结论] 1)糖尿病股动脉病变可能与JNK1的表达、JNK1磷酸化及JNK1基因的表达相关;2)中药桃仁对糖尿病股动脉病变有明显改善作用,可有效预防并抑制股动脉病变的发展进程。[Objective] Through the experimental examination Ⅱ diabetes rats JNK1 in lower extremity artery blood vessels (c-Jun N- terninal kinase,c-Jun amino terminal kinase) expression and the intervention of traditional Chinese medicine peach kernel function.[Methods] The 50 male Sprague-Dawley rats were randomly divided into blank control group,model control group,early intervention group,high-dose group and low-dose group. After successful modeling,the experimental group was given continuous drug intervention for 3 weeks,and samples were collected for experimental testing to observe the expression of various indicators.[Results] 1) The expression of JNK1 in the model group was significantly higher than that in the blank control group;2) peach kernel,a traditional Chinese medicine,can effectively reduce the expression of JNK1,significantly improve the pathological changes in the femoral artery of diabetic rats,and slow down the fibrosis process. The early intervention group has the most obvious effect,the high dose group has a good effect, and the low dose group has a poor effect.[Conclusion] 1) Diabetic femoral artery lesions may be related to JNK1 expression,JNK1 phosphorylation and JNK1 gene expression. 2) Peach kernel,a traditional Chinese medicine,can significantly improve diabetic femoral artery disease,effectively prevent and inhibit the development of femoral artery disease.

关 键 词：2型糖尿病 股动脉纤维化 桃仁 JNK1 

分 类 号：R587.1[医药卫生—内分泌]