激素组合等在甘蓝型油菜下胚轴再生中的作用  

Role of Hormone Combinations on Hypocotyls Regeneration in Brassica napus

作　　者：耿思宇 张姗姗 徐培林[2] 王景雪[1] GENG Siyu;ZHANG Shanshan;XU Peilin;WANG Jingxue(College of Life Science,Shanxi University,Taiyuan 030006,China;Key Laboratory of Gene Engineering of Ministry of Education,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]中山大学教育部基因工程重点实验室,广东广州510275

出　　处：《山西农业科学》2019年第5期730-733,760,共5页Journal of Shanxi Agricultural Sciences

摘　　要：激素和基因型对油菜外植体的高效再生具有重要作用。试验以甘蓝型油菜湘油14号、湘油15号和Y03-211共3种油菜基因型为材料,对不同浓度组合的6-BA,NAA,GA3,AgNO3组成的18种培养基在下胚轴植株再生中的作用进行研究,以明确不同激素种类在外植体再生中的作用,进而建立甘蓝型油菜下胚轴高效再生体系。结果表明,在供试的18种培养基中,所有材料都能获得80%以上的出愈率。但是不同培养基下胚轴再生率差异显著,其中,6-BA对油菜下胚轴再生植株的分化是必需的;6-BA与GA3组合优于6-BA与NAA组合;在培养基其他成分相同的条件下,添加AgNO3能够明显提高正常芽苗分化率;不同品种的再生植株分化能力不同。油菜生根使用生长素NAA比使用IAA诱导或不使用植物激素诱导的生根效果更好。基因型、激素组合以及培养基中AgNO3的添加对再生植株的分化具有重要影响,其中,MS+2 mg/L 6-BA+0.5 mg/L GA3+20μmol/L AgNO3培养基最有利于诱导再生植株分化;MS+0.2 mg/L NAA培养基最有利于诱导再生芽苗生根。Hormones and genotypes play an important role in efficient regeneration of rape explants. Three genotypes of Brassica napus, Xiangyou 14, Xiangyou 15 and Y03-211, were used as materials. The effects of 18 media composed of 6-BA, NAA, GA3 and AgNO3 with different concentration combinations on hypocotyl plant regeneration were studied, to clarify the role of different hormones in explant regeneration. Furthermore, the efficient regeneration system of hypocotyl in Brassica napus was established. The results showed that among the 18 tested media, all genotypes materials obtained more than 80% callus rate. However, the regeneration rate of hypocotyl in different media was significantly different. Among them, 6-BA was necessary for the differentiation of regenerated plants from rapeseed hypocotyl. The combination of 6-BA and GA3 was better than that of 6-BA and NAA. Adding AgNO3 could significantly improve the differentiation rate of normal buds and seedlings under the same conditions of other components in the medium. Differentiation ability of regenerated plants of different varieties was different. The inducing effect of auxin NAA on rapeseed rooting was better than IAA and no plant hormone in medium. Genotypes, hormone combinations and the addition of AgNO3 in culture medium have important effects on inducing regeneration of regenerated plants. MS+2 mg/L 6-BA+0.5 mg/L GA3+20 μmol/L AgNO3 medium is the best medium to induce regenerated plantlets, MS+0.2 mg/L NAA medium is the best medium for inducing regenerated buds to take root.

关 键 词：甘蓝型油菜 下胚轴 诱导 分化 再生植株 

分 类 号：S565.4[农业科学—作物学]