水飞蓟宾单用及合用肝龙含药血清对HSC的影响及机制研究  

Effect and Mechanism of Silybin Single-use and Combined use with Ganlong Drug-containing Serum on HSC

作　　者：吴和 杨强丽 赖泳 WU He;YANG Qiangli;LAI Yong(College of Pharmacy and Chemistry,Dali University,Dali 671000,China;Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D,Dali 671000,China)

机构地区：[1]大理大学药学与化学学院,云南大理671000 [2]云南省昆虫生物医药研发重点实验室,云南大理671000

出　　处：《中国民族民间医药》2019年第6期14-17,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：云南省教育厅科学研究基金重大专项项目(NO:ZD2014013)

摘　　要：目的:研究水飞蓟宾单用及合用肝龙含药血清对大鼠肝星状细胞增殖活性的影响及机制。方法:通过血清药理学方法制备含药血清,采用MTT法检测水飞蓟宾单用及合用肝龙含药血清对肝星状细胞增殖的影响,并用激光共聚焦测定其细胞内钙离子浓度的变化。结果:与单用水飞蓟宾相比,水飞蓟宾合用肝龙含药血清作用肝星状细胞24、48、72 h能够显著抑制肝星状细胞的增殖(P<0.01);30%水飞蓟宾联合肝龙(7.5%、15%、30%)含药血清在一定程度上可使细胞内游离Ca^(2+)浓度降低。结论:肝龙能增强水飞蓟宾在体外对肝星状细胞的抑制作用,且其作用机制可能与降低细胞内Ca^(2+)浓度有关。Objective To study the effect and mechanism of silybin single-use and combined use with ganlong capsule drug-containing serum on the proliferative activity of rat hepatic stellate cells (HSC). Methods The drug-containing serum was prepared by serum pharmacology,MTT method was used to detect the effects of silybin single-use and combined use with ganlong capsule drug-containing serum on HSC proliferation,and the changes of Ca 2+ concentration in HSC were determined via laser confocal. Results Compared with silybin single-use,silybin combined with ganlong drug-containing serum can significantly inhibit HSC proliferation at 24,48,and 72 h ( P <0.01);30% silybin combined with ganlong (7.5%,15%,30%) drug-containing serum can reduce the intracellular free Ca 2+ concentration to a certain extent. Conclusion Ganlong can enhance the inhibitory effect of silybin on HSC in vitro,and its mechanism may be related to the decrease of Ca 2+ concentration in HSC.

关 键 词：肝龙胶囊 肝纤维化 水飞蓟宾 Ca2+浓度 肝星状细胞 

分 类 号：R965[医药卫生—药理学]