光径及氮浓度对缺刻缘绿藻生长、油脂和花生四烯酸积累的影响  

EFFECTS OF LIGHT-PATHs AND NITROGEN CONCENTRATION ON THE GROWTH, TOTAL LIPIDS AND ARACHIDONIC ACID ACCUMULATION OF PARIETOCHLORIS INCISA

作　　者：印尤强 黄罗冬[2] 胡强[3,4] 张成武[2] YIN You-Qiang;HUANG Luo-Dong;HU Qiang;ZHANG Cheng-Wu(Department of Bioengineering, Jinan University, Guangzhou 510632, China;Research Center of Hydrobiology, Department ofEcology, Jinan University, Guangzhou 510632, China;Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072,China;SDIC Microalgae Biotechnology Center, China Electronics Engineering Design Institute, Beijing 100142, China)

机构地区：[1]暨南大学生物工程学系,广州510632 [2]暨南大学生态学系水生生物研究中心,广州510632 [3]中国科学院水生生物研究所,武汉430072 [4]中国电子工程设计院国投微藻生物科技中心,北京100142

出　　处：《水生生物学报》2019年第3期654-663,共10页Acta Hydrobiologica Sinica

基　　金：国家高技术研究发展计划"863"计划(2013AA065805);国家自然科学基金(31170337);广东省低碳专项(2011-051);珠海市科技重大项目(PB20041018);珠海市科技攻关项目(PC20081008);国家开发投资公司项目资助~~

摘　　要：以缺刻缘绿藻(Parietochloris incisa)为实验材料,采用BG-11培养基,分别在2种氮浓度和3种不同光径(LP)的柱状和平板光生物反应器中进行培养,并探究其生长、油脂和花生四烯酸(AA)的积累规律。结果显示:在两种光生物反应器中,光径越小,越有利于缺刻缘绿藻的生长。其中,最大生物量均在17.6 mmol/L氮浓度时获得,分别为5.09 g/L(2.5 cm-柱状)和2.98 g/L(3.0 cm-平板);而最高油脂和AA绝对含量则均在1.0 mmol/L氮浓度和最大光径处获得,分别为39.23%、13.21%(6.0 cm-柱状)和40.74%、11.33%(5.0 cm-平板);另外,两种光生物反应器中的最大油脂单位体积产率分别可以达到216.39 mg/(L·d)(17.6 mmol/L; 2.5 cm-柱状)和135.93 mg/(L·d)(1.0 mmol/L; 1.5 cm-平板);而最高的AA单位体积产率均在1.0 mmol/L低氮条件,最大光径处达到最大,分别为21.65 mg/(L·d)(6.0 cm-柱状)和19.42 mg/(L·d)(5.0 cm-平板)。因此,根据实际生产需要,在1.0 mmol/L低氮条件下,选择6.0 cm光径的柱状光生物反应器或5.0 cm光径的平板光生物反应器,培养缺刻缘绿藻生产AA,能有效降低生产成本。This study explored effects of 3 light-paths (LP) of column and flat plat photobioreactors under two kinds of nitrogen concentrations on the growth, total lipid and AA content of Parietochloris incisa using BG-11 medium. The results showed that cell growth rate increased by the smaller light-path of two kinds of photobioreactors. The maximal biomass were 5.09 g/L in column photobioreactor (2.5 cm LP) and 2.98 g/L (3.0 cm LP) in flat plate photobioreactor with nitrogen concentration at 17.6 mmol/L. The highest total lipid and AA content at the biggest length of light-path under 1.0 mmol/L nitrogen concentration were 39.23% and 13.21% in column photobioreactor (6.0 cm LP) and there were 40.74% and 11.33% in flat plate photobioreactor (5.0 cm LP). In addition, the maximal volumetric productivity of total lipid was 216.39 mg/(L d) in column photobioreactor (2.5 cm LP) under 17.6 mmol/L nitrogen concentration and it was 135.93 mg/(L d) in flat plate photobioreactor (1.5 cm LP) under 1.0 mmol/L nitrogen concentration. The AA volumetric productivity reached the maximum level in the biggest light-path of column [21.65 mg/(L d)] and flat plate photobiorector [19.42 mg/(L d)] under 1.0 mmol/L nitrogen concentration. These results indicate that the 6.0 cm light- path column photobioreactor or 5.0 cm light-path of flat plate photobioreactor under 1.0 mmol/L nitrogen concentra- tion are the best condition to culture Parietochloris incisa for AA production.

关 键 词：缺刻缘绿藻 光生物反应器 氮浓度 光径 生物量 花生四烯酸 

分 类 号：Q948.8[生物学—植物学]