太子参芜菁花叶病毒时空动态定性初步检测  

Preliminary Qualitative Detection on Spatial and Temporal Dynamics of Turnip Mosaic Virus in Radix Pseudostellariae

作　　者：赵云青[1] 黄颖桢[1] 陈菁瑛[1] 朱景花 刘保财[1] 张武君[1] ZHAO Yun-qing;HUANG Ying-zhen;CHEN Jing-ying;ZHU Jing-hua;LIU Bao-cai;ZHANG Wu-jun(Institute of Agricultural Biological Resources,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003)

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003

出　　处：《福建农业科技》2019年第3期34-39,共6页Fujian Agricultural Science and Technology

基　　金：福建省自然科学基金项目(2015J01292);福建省种业创新项目(2014S1477-12);福建省农业科学院科技创新团队项目(STIT2017-2-8);福建省农业科学院生产性工程化实验室建设项目(2015GCH-6)

摘　　要：为明确柘荣县太子参携带芜菁花叶病毒情况,采用RT-PCR法对不同太子参植株进行芜菁花叶病毒检测。结果表明:同株太子参有无病斑的叶片、根部、茎部都可检测到病毒;未表现病毒症状与表现病毒症状太子参植株的9个部位均可检测到病毒,未表现病毒症状的太子参植株中叶脉及茎下部病毒含量相对较高;不同海拔地区种植的太子参植株10个部位均可检测到病毒,花部位的病毒含量相对较少;对不同生长期太子参定株叶片进行病毒检测发现生长末期部分植株未检测到病毒,除此之外所取样品均携带芜菁花叶病毒。In order to clarify the situation of turnip mosaic virus carried by radix pseudostellariae in Zherong County, RT PCR was used to detect turnip mosaic virus in different plants of radix pseudostellariae . The results showed that viruses in could be detected in leaves, roots and stems of the same plant with or without disease spots. Viruses Radix pseudostellariae could be detected in 9 parts of plants with and without viral symptoms, while the content of viruses in middle veins and lower stems of plants without viral symptoms was relatively higher. Viruses in Radix pseudostellariae could be detected in 10 parts of plants at different altitudes, but the virus content in flower parts was relatively lower. Virus detection on the leaves of fixed plants of radix pseudostellariae at different growth stages showed that no virus was detected in some plants at the end of growth stage. In addition, all the samples carried turnip mosaic virus.

关 键 词：柘荣县 太子参 芜菁花叶病毒 RTPCR 检测 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]