5种条件下桉树焦枯病菌MAPK基因的表达  

Expression profiles of MAPK genes in Calonectria pseudoreteaudii under 5 conditions

作　　者：杨泽慧[1] 陆芝 李丽红 江仲鹏 冯锦秀 冯丽贞[1] YANG Zehui;LU Zhi;LI Lihong;JIANG Zhongpeng;FENG Jinxiu;FENG Lizhen(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学林学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2019年第3期337-343,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建农林大学科技创新专项(KFA17053A);福建农林大学科技创新专项基金项目(KFA17278A);福建省财政厅资助项目(K81150002)

摘　　要：采用qPCR检测Calonectria pseudoreteaudii YA51的MAPK基因在盐胁迫、氧胁迫、细胞壁干扰物质胁迫、桉树组织诱导以及不同发育时期5种条件下的表达情况.结果表明CpKss1参与致病过程,CpKss1、CpSlt2和CpIme2与菌丝生长发育有关,CpKss1、CpHog1和CpIme2参与病菌高渗胁迫反应,CpSlt2参与调控氧胁迫反应和细胞壁完整性,且4个MAPK基因所介导的级联通路间可能存在既相互独立又相互协作的关系.Expression of MAPK genes of Calonectria pseudoreteaudii YA51 under salt stress, H2O2 stress, CFW stress, infection, and at different developmental stages were quantatively analyzed by qPCR. According to the expression levels, CpKss1 is likely to be involved in the regulation of pathogenicity;CpKss1, CpSlt2 and CpIme2 are involved in the mycelial growth and development;CpKss1, CpHog1 and CpIme2 participate under hypertonic condition;CpSlt2 is involved in the maintaining of cell wall integrity and the regulation under H2O2 stress. Furthermore, there were mutually independent and cooperative relationships in the signaling cascades mediated by the 4 genes.

关 键 词：桉树焦枯病菌 MAPK qPCR 

分 类 号：S763.15[农业科学—森林保护学]