莫西沙星可浓度依赖性增加人气道平滑肌细胞Caveolin-1的表达  

Moxifloxacin Increasing the Expression of Caveolin-1 of Human Airway Smooth Muscle Cell in Concentration-dependent Manner

作　　者：李慧婷 赵杰 李海泉 施萍 张衍民 杜永亮 LI Hui-ting;ZHAO Jie;LI Hai-quan;SHI Ping;ZHANG Yan-min;DU Yong-liang(The Second Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu Prvoince,221006 China)

机构地区：[1]徐州医科大学第二附属医院呼吸内科,江苏徐州221006

出　　处：《中外医疗》2019年第9期11-13,18,共4页China & Foreign Medical Treatment

基　　金：徐州市科技局课题(KC16SL102)

摘　　要：目的观察在不同浓度的莫西沙星作用下,人气道平滑肌细胞(HASMC)小凹蛋白-1(Caveolin-1,Cav-1)蛋白及mRNA表达的变化,探讨莫西沙星对HASMC发挥抗炎调节的可能机制。方法购买HASMC,体外传代培养,按随机数字表法分为空白对照组(M0)、莫西沙星5 mg/L(M5组)、10 mg/L(M10组)、20 mg/L(M20组),均孵育48 h后,分别应用Western blot和qRT-PCR检测Cav-1蛋白水平及mRNA表达。结果 Western blot检测显示,各莫西沙星浓度组(M5组、M10组、M20组)Cav-1蛋白表达分别为(3.02±0.43),(4.18±0.07),(4.44±0.14),均明显高于空白对照组(M0组)(1.00±0.15)(P<0.05),同时也存在浓度依赖性(r=0.77)。qRT-PCR结果显示各莫西沙星组(M5组、M10组、M20组)Cav-1 mRNA表达量(2.54±0.35),(3.26±0.22),(5.57±0.51)均较空白对照组(M0组)表达量(1.00±0.11)明显增加(P<0.05),同时存在浓度依赖性(r=0.99)。结论莫西沙星可浓度依赖性增加HASMC Cav-1蛋白及m RNA表达,这可能与莫西沙星对ASMC发挥抗炎作用相关。Objective To culture human airway smooth muscle cells(HASMCs)with moxifloxacin at various concentrations,then to observe the effects of moxifloxacin on the expression of the protein and m RNA of Cav-1,and to explore the possible mechanisms of anti-inflammatory effects of moxifloxacin on HASMCs.Methods HASMCs were bought and cultured in vitro.The cells were randomly divided into 4 groups including a control group(M0)and 3 groups to which moxifloxacin was added at different concentrations(5,10,20)mg/L,groups M5,M10 and M20 respectively).Then the cells of different groups were incubated for 48 h.Finally the protein of Cav-1 was measured by Western blot and the mRNA of Cav-1 was measured by q RT-PCT respectively.Results The results of Western blot indicated that the expression of Cav-1 increased with the concentrations of moxifloxacin increasing(3.02±0.43),(4.18±0.07),(4.44±0.14)respectively,and the expression of Cav-1 of these groups were all higher than that of the control group(1.00±0.15)(P<0.05),The expression of Cav-1 showed a positive correlation with the concentration of moxifloxacin(r=0.77).The results of q RT-PCR showed that the expression of mRNA of Cav-1 of different moxifloxacin groups M5,M10 and M20(2.54±0.35),(3.26±0.22),(5.57±0.51)respectively were all higher than that of the control group(1.00±0.11)(P<0.05).There was also a positive correlation between the m RNA of Cav-1 and the concentration of moxifloxacin(r=0.99).Conclusion Moxifloxacin could increase the expression of Caveolin-1 of HASMCs in concentration-dependent manner,which may be the possible mechanism concerning the anti-inflammatory effects of moxifloxacin.

关 键 词：莫西沙星 肌细胞 平滑肌 小凹蛋白-1 

分 类 号：R562.2[医药卫生—呼吸系统]