叶酸添加水平对肉仔鸡肝脏中脂肪代谢相关基因表达和甲基化的影响  

Effects of Folate Supplemental Level on Expression and Methylation of Lipid Metabolism Related Genes in Liver of Broilers

作　　者：邢晋祎[1] 张渝洁[1] 张宁波 井文倩 李珠 王新正 徐云超 XING Jinyi;ZHANG Yujie;ZHANG Ningbo;JING Wenqian;LI Zhu;WANG Xinzheng;XU Yunchao(School of Life Sciences,Linyi University,Linyi 276005,China;College of Agriculture and Forestry Sciences,Linyi 276005,China)

机构地区：[1]临沂大学生命科学学院,临沂276005 [2]临沂大学农林科学学院,临沂276005

出　　处：《动物营养学报》2019年第5期2098-2106,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31372333);山东省自然科学基金项目(ZR2017LC018)

摘　　要：本试验旨在考察叶酸添加水平对肉仔鸡血清中脂蛋白脂肪酶(LPL)和脂肪酸合成酶(FAS)活性、过氧化物酶体增殖物激活受体γ(PPARγ)含量以及肝脏中脂肪代谢相关基因表达和甲基化的影响。选择1日龄爱拔益加(AA)肉仔鸡300只,随机分成3组,每组5个重复,每个重复20只。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加5和10 mg/kg叶酸的试验饲粮。试验期为42 d。结果显示:1)与对照组相比,饲粮中添加10 mg/kg叶酸显著降低了21日龄肉仔鸡血清中LPL活性(P<0.05);饲粮中添加5和10 mg/kg叶酸极显著降低了42日龄肉仔鸡血清中LPL活性(P<0.01),极显著提高了21和42日龄肉仔鸡血清中PPARγ含量(P<0.01),极显著降低了21和42日龄肉仔鸡血清中FAS活性(P<0.01)。2)实时荧光定量PCR结果发现,饲粮中添加5和10 mg/kg叶酸均未显著影响42日龄肉仔鸡肝脏中LPL和PPARγ基因的相对表达量(P>0.05),但均显著降低了FAS基因的相对表达量(P <0.05)。3)甲基化分析显示,饲粮中添加5和10 mg/kg叶酸均未显著影响42日龄肉仔鸡肝脏中LPL和PPARγ基因的甲基化比率(P>0.05),但饲粮中添加5 mg/kg叶酸极显著提高了42日龄肉仔鸡肝脏中FAS基因的甲基化比率(P<0.01)。由此得出,饲粮中添加5和10 mg/kg叶酸极显著降低了42日龄肉仔鸡血清中LPL和FAS的活性,极显著增加了PPARγ的含量,显著降低了肝脏中FAS基因的表达;此外,饲粮中添加5 mg/kg叶酸还极显著提高了42日龄肉仔鸡肝脏中FAS基因的甲基化水平。The aims of this study were to investigate the effects of different folate supplemental levels on the activities of lipoprotein lipase(LPL),fatty acid synthase(FAS)and peroxisome proliferator-activated receptor gamma(PPARγ)content in serum and the expression and methylation of lipid metabolism related genes in liver of broilers.A total of 300 one-day-old Arbor Acres(AA)broilers were randomly allotted to 3 groups with 5 replicates of 20 broilers each.Broilers in control group were fed a basal diet,and broilers in trial groups were fed the basal diet supplemented with 5 and 10 mg/kg folate,respectively.The experiment lasted for 42 days.The results showed as follows:1)compared with the control group,diet supplemented with 10 mg/kg folate significantly reduced the activity of LPL in serum of 21-day-old broilers(P<0.05);diet supplemented with 5 and 10 mg/kg folate significantly reduced the activity of LPL in serum of 42-day-old broilers(P<0.01),significantly increased the content of PPARγin serum of 21-and 42-day-old broilers(P<0.01),and significantly reduced the activity of FAS in serum of 21-and 42-day-old broilers(P<0.01).2)The results of real-time quantitative PCR indicated that the relative expression levels of LPL and PPARγgenes in liver of 42-day-old broilers were not significantly affected by diet supplemented with 5 and 10 mg/kg folate(P>0.05).Compared with the control group,the relative expression levels of FAS gene in liver of 42-day-old broilers was significantly decreased by diet supplemented with 5 and 10 mg/kg folate(P<0.05).3)The results of methylation analysis showed that the methylation ratios of LPL and PPARγgenes in liver of 42-day-old broilers were not significantly affected by diet supplemented with 5 and 10 mg/kg folate(P>0.05).Compared with the control group,diet supplemented with 5 mg/kg folate significantly improved the methylation ratio of FAS gene in liver of 42-day-old broilers(P<0.01).In conclusion,diet supplemented with 5 and 10 mg/kg folate can significantly decrease serum LPL and F

关 键 词：肉仔鸡 叶酸 甲基化 脂蛋白脂肪酶 过氧化物酶体增殖物激活受体Γ 脂肪酸合成酶 

分 类 号：S816[农业科学—饲料科学]