丹连消痤散外用对痤疮模型兔耳组织IL-6 IL-8表达影响的实验研究  

Experimental Study on Effects of Danlian Xiaocuo Powder(丹连消痤散)External Application on IL-6 and IL-8 Expressions in Rabbits' Ears with Acne

作　　者：贾丽梅 谢碧 JIA Limei;XIE Bi(Heilongjiang Academy of Chinese Medical Sciences, Harbin,Heilongjiang 150036)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150036

出　　处：《中国中医药科技》2019年第3期343-346,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：哈尔滨市科学技术局研究项目(2017RAXQJ083)

摘　　要：目的:观察丹连消痤散外用对痤疮模型兔耳组织病理变化及IL-6、IL-8表达的影响。方法:将48只兔随机分为6组,正常对照组,模型组,阳性对照组(克痤隐酮凝胶)和丹连消痤散高、中、低剂量组,每组8只;除正常对照组外其余5组造模成功后进行相应干预,连续给药14 d。观察兔耳局部皮肤病理变化及免疫组化法检测耳组织IL-6和IL-8的表达。结果:各给药组兔耳局部病理变化较模型组均明显减轻。与正常组相比,其余5组兔耳组织IL-6、IL-8表达均显著提高;与模型组相比,阳性对照组与丹连消痤散3剂量组而组织IL-6、IL-8表达显著降低,并且中剂量组效果最为显著,IL-8变化与阳性组比较差异有统计学意义(P<0.05)。结论:丹连消痤散外用可有效减轻兔耳痤疮病理变化,降低IL-6、IL-8表达是其部分作用机制。Objective: To observe the effects of Danlian Xiaocuo Powder on pathological changes of rabbits’ ears with acne and IL-6, IL-8 expressions in ears. Methods: 48 Rabbits were randomly divided into 6 groups, normal, model, positive and Danlian Xiaocuo Powder low, middle,high dose groups, the other 5 groups, except the normal group, were given intervention for 14 days after successful modeling. Observing the pathological changes of the models’ ears and detected the expressions of IL-6 and IL-8 in rabbits ‘ ears with acne by immunohistochemical method. Results: To compare with model group, the pathological changes of each medicating group rabbits’ ears with acne were alleviated significantly. To compare with the normal group, the expressions of IL-6 and IL-8 in the other five groups rabbits’ ears were significantly increased, compared with the model group, the expressions of IL-6 and IL-8 in the three dose of Danlian Xiaocuo Powder groups and the positive control group were significantly decreased, and the most significant was seen in the middle-dose group, there was significant difference between the middle dose group and positive group in IL-8 expression( P <0.05). Conclusion: Danlian Xiaocuo Powder external application can effectively reduce the pathological changes of rabbits’ ears with acne, lowering the expressions of IL-6 and IL-8 in the part of mechanism.

关 键 词：痤疮 丹连消痤散 克痤隐酮凝胶 病理变化 白细胞介素6 白细胞介素8 兔 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]