Periostin对心肌梗死后中性粒细胞浸润及心肌修复的调控作用研究  

The role of periostin in regulating neutrophil infiltration and cardiac repair after myocardial infarction

作　　者：祝双华 廖延 姜美花[2] 詹宇婷 彭朝权[1] Zhu Shuanghua;Liao Yan;Jiang Meihua;Zhan Yuting;Peng Chaoquan(Department of Cardiovascular Disease,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院心血管内科,广州510630 [2]中山大学干细胞与组织工程研究中心,广州510060 [3]中山大学附属肿瘤防治中心,广州510800

出　　处：《新医学》2019年第5期353-360,共8页Journal of New Medicine

基　　金：国家自然科学基金(81370214);广东省自然科学基金(2015A030313183)

摘　　要：目的探讨心肌梗死后periostin对心功能的影响及其是否通过调控中性粒细胞发挥作用,为临床治疗提供一定理论基础。方法分别取正常野生型(WT)和periostin敲除(periostin^-/-)C57小鼠各60只,通过心脏左前降支永久结扎的方式制备心肌梗死模型,在心肌梗死前及心肌梗死后3周检测2组小鼠心功能指标,2,3,5-氯化三苯基四氮唑(TTC)染色检测组织形态学变化,并记录2组小鼠生存情况;在心肌梗死后第1、3日通过流式方式检测2组小鼠心肌梗死区中性粒细胞聚集情况。结果心脏彩色多普勒超声显示心肌梗死前2组小鼠的LVEF、左心室缩短分数(LVFS)、左心室收缩末容积(LVESV)、左心室舒张末容积(LVEDV)比较差异均无统计学意义(P均>0.05),而心肌梗死后3周periostin^-/-组LVEF及LVFS高于WT组(P均<0.01),LVESV及LVEDV均低于WT组(P均<0.05)。TTC染色示periostin^-/-组梗死灶面积较WT组更小(t=5.817,P<0.05)。Periostin^-/-组的生存率高于WT组(log-rank P=0.046)。流式结果显示心肌梗死后第1日periostin^-/-组心肌梗死区中性粒细胞数量多于WT组(t=5.365,P<0.01),但在第3日少于WT组(t=2.548,P<0.05)。结论心肌梗死后periostin可以抑制中性粒细胞进入梗死区清除坏死心肌,不利于后续心脏的修复以及心功能的改善。Objective To evaluate the effect of periostin on the cardiac function after myocardial infarction and investigate whether it plays a role by regulating the neutrophil infiltration,aiming to provide a theoretical basis for clinical treatment.Methods The mouse models of myocardial infarction(MI)were established by permanent ligation of the left anterior descending branch using wild-type(WT)C57 mice(n = 60)and periostin^-/-C57 mice(n = 60).Before and 3 weeks after MI,the parameters related to cardiac function were assessed.The histological and morphological changes were observed by 2,3,5-triphenyltetrazole chloride(TTC)staining.The survival rate was recorded in two groups.At 1 and 3 d after MI,the neutrophil aggregation and expression levels of inflammatory factors were detected by flow cytometry and qPCR.Results Echocardiography demonstrated that the left ventricular ejection fraction(LVEF),left ventricular fraction shortening(LVFS),left ventricular end-systolic volume(LVESV),left ventricular end-diastolic volume(LVEDV)did not significantly differ between the WT and periostin^-/- groups before MI(all P>0.05),whereas the LVEF and LVFS of the periostin^-/- mice were significantly higher than those of WT mice at 3 weeks after MI(both P<0.01)and both LVESV and LVEDV of the periostin^-/- mice were considerably lower than those of WT mice(both P<0.05).TCC staining revealed that the area of infarction lesions in the periostin^-/- group was significantly smaller than that in the WT group(t = 5.817,P<0.05).Compared with the WT mice,the survival rate of periostin^-/- mice was significantly higher at 3 weeks after MI(log-rank P = 0.046).Flow cytometry revealed that the quantity of neutrophils in the infarction area of periostin^-/- mice was considerably higher at 1 d after MI(t = 5.365,P<0.01),whereas significantly lower at 3 d following MI(t = 2.548,P<0.05)compared with those of WT mice.Conclusion Periostin can suppress the neutrophils from infiltrating into the infarction area to eliminate the necrotic myocardium,which is n

关 键 词：心肌梗死 PERIOSTIN 中性粒细胞 心肌修复 

分 类 号：R542.22[医药卫生—心血管疾病]