内窥镜引导气管注入博来霉素建立小鼠肺纤维化模型  

Endoscopy Guided Tracheal Injecting Bleomycin Model of Pulmonary Fibrosis in Mice

作　　者：余华军 吴尚 黄慧 林碧云 伍俊 欧华俊 张海涛 YU Hua-jun;WU Shang;HUANG Hui;LIN Bi-yun;WU Jun;OU Hua-jun;ZHANG Hai-tao(Experimental Animal Center,Guangdong Medical University,524023,China;Institute of Biochemistry and Molecular Biology,Guangdong Medical University,Zhanjiang 524023,China;Department of Respiratory Medicine,Affiliated Hospital,Guangdong Medical University,Zhanjiang 524001,China)

机构地区：[1]广东医科大学实验动物中心,湛江524023 [2]广东医科大学生物化学与分子生物学教研室,湛江524023 [3]广东医科大学附属医院呼吸内科,湛江524001

出　　处：《实验动物与比较医学》2019年第2期94-98,共5页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金(81772634;81770034);广东医科大学科研基金(2XK16042);湛江市非资助科技攻关计划项目(2017B01064)

摘　　要：目的建立在内窥镜直视下气管插管法,并采用此方法建立博来霉素诱导小鼠肺纤维化模型。方法将5只C57BL/6J小鼠,在内窥镜直视下进行小鼠气管插管,建立气管插管方法及步骤。将10只C57BL/6J小鼠随机均分为对照组和实验组,按照建立的方法进行气管给药,对照组气管插管给予50μL的生理盐水,实验组气管插管给予50μL 3.5 mg/kg的博来霉素,观察各组小鼠的死亡率。灌注后28 d,颈椎脱臼法处死小鼠,比较两组小鼠肺组织谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)活性和羟脯胺酸(HYP)、丙二醛(MDA)含量。取小鼠肺组织进行病理切片,HE、Masson染色观察肺组织的病理情况。结果 15只C57BL/6J小鼠采用多功能内窥镜直视下气管插管均能成功,无小鼠因插管后死亡,5只给予印度墨汁的预实验小鼠气管下端开始直至肺部均有黑色物质均匀分布。与对照组比较,实验组小鼠肺组织中HYP、MDA含量增高(P<0.01); GSH-PX、SOD含量降低(P<0.01)。进行HE、Masson染色显示,与对照组比较,实验组小鼠肺组织胶原沉积严重,病理学表现符合肺纤维化改变。结论采用多功能内窥镜直视下气管给药的方法,给药量准确、安全、简便。以此方法建立博来霉素诱导小鼠肺纤维化的模型成功率高,值得推广应用。Objective To establish intratracheal intubation under direct vision of multifunctional endoscopic, and adopted to establish the model of bleomycin induced pulmonary fibrosis in C57 BL/6 J mice. Methods Establish method and steps of endotracheal intubation under direct vision of multifunctional endoscopic with five C57 BL/6 J mice. Ten mice were randomly divided into two groups(5 in each). Control group were given 50 μL of saline and experimental group were given 50 μL of bleomycin(3.5 mg/kg) to observe the death rates of mice. At the 28 th day, the mice were sacrificed by cervical delocation. By compared the vitality of superoxide dismutase(SOD), glutathione peroxidase(GSH-PX), the content of malondialdehyde(MDA) and hydroxyproline(HYP) in the two groups of mouse lung tissue. Lung tissue fibrosis degree were observed by HE and Masson stain. Results All C57 BL/6 J mice were successfully intubated by using multifunctional endoscopy under direct view and no mice died after intubation. The 5 mice given India ink had black materials evenly distributed from trachea bottom until the lungs. Compared with those of control group, HYP and MDA contents in mouse lung tissue experimental group elevated(P<0.01), while SOD and GSH-PX level in mice lung tissue decreased(P<0.01). The lung tissue was obtained and stained by HE and Masson. By comparison with control group, collagen deposition in experimental group mouse lung tissue is serious. The pathological changes were in accordance with pulmonary fibrosis. Conclusion The method of endotracheal intubation under direct vision of multifunctional endoscopic is safe, effective and convenient. This method was successful to establish pulmonary fibrosis in mice model with high efficiency induced by bleomycin,and worthy of popularization and application.

关 键 词：内窥镜 气管插管 肺纤维化 动物模型 

分 类 号：Q95-33[生物学—动物学]