鲜天南星水提取物对小鼠子宫纤维瘤的抑制作用和对小鼠雌激素的影响  

Inhibition of uterine fibroids by the extract of fresh Tiannanxing and its effect on estrogen in mice

作　　者：董微[1] 张博[2] 邵超[1] DONG Wei;ZHANG Bo;SHAO Chao(Department of Gynaecology,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 102600,China;Department of Acupuncture and Moxibustion,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 102600,China)

机构地区：[1]中国中医科学院广安门医院南区妇科,北京102600 [2]中国中医科学院广安门医院南区针灸科,北京102600

出　　处：《西部医学》2019年第5期679-682,688,共5页Medical Journal of West China

摘　　要：目的探讨鲜天南星水提取物对小鼠子宫纤维瘤的抑制作用及对小鼠雌激素水平的影响。方法选取SD健康雌性小鼠30只,随机分为正常组、模型组、实验组3组,每组各10只,对模型组、实验组进行子宫纤维瘤模型建立,正常组不作处理。实验组小鼠予以鲜天南星水提取物进行干预,正常组、模型组小鼠注射等体积生理盐水。对小鼠子宫重及子宫系数进行统计计算,酶联免疫吸附试验法检测雌二醇(Estradiol,E2)、孕酮(Progesterone,P)、卵泡刺激素(Follicle-stimulating hormone,FSH)、黄体生成激素(Luteinizing Hormone,LH)水平。结果干预后,模型组及实验组小鼠子宫重及子宫系数均高于正常组,实验组小鼠子宫重及子宫系数均低于模型组(P<0.05);干预后,模型组及实验组小鼠血清E2、P、FSH、LH水平均高于正常组,但实验组小鼠血清E2、P、FSH、LH水平均低于模型组(P<0.05)。结论鲜天南星水提取物可对小鼠子宫纤维瘤起到一定的抑制作用,并能对E_2、P、FSH、LH等激素水平进行调控,为子宫纤维瘤的临床治疗提供一定的理论依据,值得临床借鉴。Objective To investigate the inhibitory effect of fresh Tiannanxing water extract on uterine fibroids of mice and the effect on estrogen level of mice.Methods Thirty healthy female SD mice were selected,and randomly divided into normal group,model group and fresh Tiannanxing water extract group.The model group and fresh Tiannanxing water extract group were established for uterine fibroids model,while the normal group was not treated.Rats in the fresh Tiannanxing water extract group were given an intervention.The rats in the normal group and model group were injected with normal saline.The weight and mouse uterus coefficient,chemiluminescence detection of Estradiol (E 2),Progesterone (P),enzyme-linked immunosorbent assay method for detecting FSH (FSH),Luteinizing hormone generated (LH) level were analyzed.Results The uterine weight of mice in the model group was higher than that in the normal group,and that in the fresh Tiannanxing water extract group was higher than that in the normal group,lower than that in the model group ( P <0.05).The uterus coefficient of mice in the model group was higher than that in the normal group,and that in the fresh Tiannanxing water extract group was higher than that in the normal group,lower than that in the model group ( P <0.05).The serum E 2 level of mice in the model group was higher than that in the normal group,and the serum E 2 level of mice in the fresh Tiannanxing water extract group was higher than that in the normal group,lower than that in the model group ( P <0.05).The serum P level of mice in the model group was higher than that in the normal group,and that in the fresh Tiannanxing water extract group was higher than that in the normal group and lower than that in the model group ( P <0.05).The serum FSH level of mice in the model group was higher than that in the normal group,and the serum FSH level of mice in the fresh Tiannanxing water extract group was higher than that in the normal group,lower than that in the model group ( P <0.05).The serum LH level in the mod

关 键 词：子宫纤维瘤 鲜天南星水提取物 雌激素 子宫系数 

分 类 号：R285.5[医药卫生—中药学]