高脂血症大鼠AQP3和AQP4表达及泽泻汤加味方干预作用  

Expression of AQP3 and AQP4 in colonic tissues of rats with hyperlipidemia and intervention effect of modified Rhizoma Alismatis decoction

作　　者：段思明 韩雪[1] 张睦清 张一昕[1] 王茜[1] 王亚芬[1] 刘晨旭 DUAN Si-ming;HAN Xue;ZHANG Mu-qing(Hebei University of Chinese Medicine,Shijiazhuang,Hebei Province 050200,China)

机构地区：[1]河北中医学院,河北石家庄050200 [2]河北省中医院河北中医学院附属医院

出　　处：《中国公共卫生》2019年第4期406-409,共4页Chinese Journal of Public Health

基　　金：河北省中医院管理局科学技术研究计划资助项目(2016008);河北省高等学校科学技术研究项目(QN2015251)

摘　　要：目的观察泽泻汤加味方对高脂血症大鼠血脂及结肠组织水通道蛋白3和4(AQP3、AQP4)基因、蛋白表达的影响。方法将60只雄性SD大鼠随机分为对照组、模型组、阳性对照组(辛伐他汀)及泽泻汤加味方高、中、低剂量组,采用喂饲高脂饲料方法复制大鼠模型,造模同时给予相应药物干预,连续5周;观察大鼠血清中甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)含量变化,运用RT-PCR、Western blot方法检测大鼠结肠组织中AQP3和AQP4基因、蛋白表达情况。结果与对照组比较,模型组大鼠血清中TG、TC、LDL-C含量[分别为(2.183±0.204)、(2.013±0.250)、(1.267±0.087)mmol/L]升高,HDL-C含量[(0.731±0.085)mmol/L]降低(P <0.05);与模型组比较,高剂量泽泻汤加味方组大鼠血清中TG、TC、LDL-C含量[分别为(1.205±0.139)、(1.164±0.197)、(0.787±0.057)mmol/L]下降,HDL-C含量[(0.904±0.115)mmol/L]升高(P <0.05)。与对照组比较,模型组大鼠结肠组织中AQP3和AQP4蛋白表达水平[分别为(0.944±0.052)、(0.701±0.067)]升高(P <0.01);与模型组比较,高剂量泽泻汤加味方组大鼠结肠组织中AQP3和AQP4蛋白表达水平[(0.502±0.085)、(0.366±0.061)]均降低,呈剂量效应关系(P <0.05)。结论泽泻汤加味方具有调节血脂作用,其机制可能与结肠组织AQP3和AQP4蛋白表达调控有关。Objective To examine effects of Rhizoma Alismatis decoction (RAD) on hyperlipidemia and expressions of aquaporin 3 (AQP3) and aquaporin 4 (AQP4) in colon tissues in rats. Methods Sixty male Sprague-Dawley rats were randomly divided into 6 groups: a blank and a model group with distilled water,a positive control group with simvastatins, and high-,moderate-,and low-dose groups with modified RAD by gavage once a day continuously for five weeks. All the rats were fed with high-fat diet except for those of the blank control with normal diet. At the end of the treatments,serum total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C),and low-density lipoprotein cholesterol (LDL-C) were detected and the expressions of AQP3 and AQP4 in colon tissues were detected with real-time reverse transcription PCR (RT-PCR) and Western blot. Results Compared to those in the blank control rats,serum TG (2.183 ± 0.204 mmol/L),TC (2.013 ± 0.250 mmol/L),LDL-C (1.267 ± 0.087 mmol/L) were significantly elevated but serum HDL-C (0.731 ± 0.085 mmol/L) was significantly reduced in in the model rats (P < 0.05 for all). Compared to those in the blank control rats,serum TG (1.205 ± 0.139 mmol/L),TC (1.164 ± 0.197 mmol/L),LDL-C (0.787 ± 0.057 mmol/L) were decreased but serum HDL-C (0.904 ± 0.115 mmol/L) was increased significantly in the rats with high-dose modified RAD (P < 0.05 for all). The expressions of AQP3 (0.944 ± 0.052) and AQP4 (0.701 ± 0.067) in colon tissues of the model rats increased significantly compared to those of the blank control rats (both P < 0.01);while,the expressions of AQP3 (0.502 ± 0.085) and AQP4 (0.366 ± 0.061) in colon tissues of the rats treated with high-dose modified RAD decreased significantly compared to those of the model rats (both P < 0.05). Conclusion Modified Rhizoma Alismatis decoction can regulate blood lipids in rats and the effect may be related to the regulated expressions of AQP3 and AQP4 in colon tissue.

关 键 词：泽泻汤加味方 高脂血症 水通道蛋白3(AQP3) 水通道蛋白4(AQP4) 

分 类 号：R285.5[医药卫生—中药学]